Département PIV

Epithelia ciliés – transport et auto-organisation

Responsable : E. Loiseau

Présentation

Physics of the mucociliary clearance.

Principal investigators: E. Loiseau, A. Viallat,
Postdoc: O. Mesdjian
PhD student: A. Baby

Topology and dynamics of ciliated epithelia : fluid flow patterns and biological functions

From swimming microorganisms to major organs including the brain, the respiratory system and the reproductive tracts, ciliated cells are ubiquitous in living systems. Their multiple active cilia continuously consume chemical energy and beat unidirectionally to ensure motility of marine microorganisms, circulation of the cerebrospinal fluid in the central nervous system, mucociliary clearance of pathogens and pollutants from airways, flow generation to clear the skin of Xenopus embryo from pathogens. The generation of fluid flows at the tissue level requires a dense array of cilia that coordinate their beat directions at long range. Furthermore, cilia are exposed by ciliated cells in tight adhesion with their neighbors to form a connective tissue which has its own dynamics and constraints.

We focus on two main systems: the bronchial epithelium and Xenopus embryo. To identify the physical and biological mechanisms that govern the self-organisation of cilia and the patterning of the underlying tissue we combine topological active matter, cell biology and numerical simulations.

We develop new  methods and analysis tools, based on advanced optical microscopy and image processing, in order to quantify the mechanics, the hydrodynamics and the dynamic processes of ciliated epithelia, from the cellular scale to the tissue level, and up to an entire organism. We aim at identifying biophysical markers in respiratory diseases.

 

The airway epithelium is protected by the active transport at its surface of a layer of a complex fluid, the mucus. It is powered by the beats of billions of microscopic cilia carried by epithelial cells. These beats present a strong long-range orientational order but the physical mechanisms leading to the emergence and maintain of this collective dynamic order of ciliary beats is not known. They involve the mechanical cilia-mucus coupling mediated by long-range hydrodynamic interactions, and the active mechanotransductive response of ciliary beats. We study the long-range self-organization of ciliary activity and mucus flow patterns in reconstituted bronchial epithelium.

 

 

Publications

2020

Mechanical adaptation of monocytes in model lung capillary networks

Jules Dupire, Pierre-Henri Puech, Emmanuèle Helfer, Annie Viallat

Proceedings of the National Academy of Sciences of the United States of America 117:14798 (2020)

Hydrodynamic model of directional ciliary-beat organization in human airways

Simon Gsell, Etienne Loiseau, Umberto D’ortona, Annie Viallat, Julien Favier

Scientific Reports 10 (2020)10.1038/s41598-020-64695-w

Femtomolar detection of Cu2+ ions in solution using super-Nernstian FET-sensor with a lipid monolayer as top-gate dielectric

A. Kenaan, F. Brunel, J.-M. Raimundo, Anne Charrier

Sensors and Actuators B: Chemical 316:128147 (2020)10.1016/j.snb.2020.128147

Active mucus–cilia hydrodynamic coupling drives self-organization of human bronchial epithelium

Etienne Loiseau, Simon Gsell, Aude Nommick, Charline Jomard, Delphine Gras, Pascal Chanez, Umberto D’ortona, Laurent Kodjabachian, Julien Favier, Annie Viallat

Nature Physics (2020)10.1038/s41567-020-0980-z

Electrolyte-gated-organic field effect transistors functionalized by lipid monolayers with tunable pH sensitivity for sensor applications

Tin Phan Nguy, Ryoma Hayakawa, Volkan Kilinc, Matthieu Petit, Yemineni S L V Narayana, Masayoshi Higuchi, Jean-Manuel Raimundo, Anne Charrier, Yutaka Wakayama

Applied Physics Express 13:011005 (2020)10.7567/1882-0786/ab5322

Molecular and Mechanobiological Pathways Related to the Physiopathology of FPLD2

Alice-Anaïs Varlet, Emmanuèle Helfer, Catherine Badens

Cells 9:1947 (2020)10.3390/cells9091947

2019

Self-organization of red blood cell suspensions under confined 2D flows

Cécile Iss, Dorian Midou, Alexis Moreau, Delphine Held, Anne Charrier, Simon Mendez, Annie Viallat, Emmanuèle Helfer

Soft Matter (2019)10.1039/C8SM02571A

Novel and innovative interface as potential active layer in Chem-FET sensor devices for the specific sensing of Cs

Volkan Kilinc, Catherine Henry-De-Villeneuve, Tin Phan Nguy, Yutaka Wakayama, Anne Charrier, Jean-Manuel Raimundo

ACS Applied Materials & Interfaces 11:47635-47641 (2019)10.1021/acsami.9b18188

Stable operation of water-gated organic field-effect transistor depending on channel flatness, electrode metals and surface treatment

Tin Phan Nguy, Ryoma Hayakawa, Volkan Kilinc, Matthieu Petit, Jean-Manuel Raimundo, Anne Charrier, Yutaka Wakayama

Japanese Journal of Applied Physics 58:SDDH02 (2019)10.7567/1347-4065/ab09d2

2018

Nanometrology of Biomass for Bioenergy: The Role of Atomic Force Microscopy and Spectroscopy in Plant Cell Characterization

Anne Charrier, Aude Lereu, Rubye Farahi, Brian Davison, Ali Passian

Frontiers in Energy Research 6:11 (2018)10.3389/fenrg.2018.00011

Reconstitution of composite actin and keratin networks in vesicles

J. Deek, R. Maan, E. Loiseau, R. Bausch

Soft Matter 14:1897-1902 (2018)10.1039/c7sm00819h

Facile Nanopatterning of PEDOT:PSS Thin Films

Mohammed Elmahmoudy, Anne Charrier, George Malliaras, Sébastien Sanaur

Advanced Materials Technologies 3:1700344 (2018)10.1002/admt.201700344

Ultra-thin supported lipid monolayer with unprecedented mechanical and dielectric properties

Ahmad Kenaan, Racha El Zein, Volkan Kilinc, Sébastien Lamant, Jean-Manuel Raimundo, Anne Charrier

Advanced Functional Materials 1801024 (2018)10.1002/adfm.201801024

Spatiotemporal organization of cilia drives multiscale mucus swirls in model human bronchial epithelium

Mustapha-Kamel Khelloufi, Etienne Loiseau, Marc Jaeger, Nicolas Molinari, Pascal Chanez, Delphine Gras, Annie Viallat

Scientific Reports 8:2447 (2018)10.1038/s41598-018-20882-4

Adhesion of Active Cytoskeletal Vesicles

Renu Maan, Etienne Loiseau, Andreas Bausch

Biophysical Journal 115:2395-2402 (2018)10.1016/j.bpj.2018.10.013

When giant vesicles mimic red blood cell's dynamics: swinging of two-phase vesicles in shear flow

Simon Tusch, Etienne Loiseau, Al-Hair Al-Halifa, Kamel Khelloufi, Emmanuèle Helfer, Annie Viallat

Physical Review Fluids 3 (2018)10.1103/PhysRevFluids.3.123605

2017

Printing functional protein nano-dots on soft elastomers: from transfer mechanism to cell mechanosensing

Ranime Alameddine, Astrid Wahl, Fuwei Pi, Kaoutar Bouzalmate, Laurent Limozin, Anne Charrier, Kheya Sengupta

Nano Letters 17:4284-4290 (2017)10.1021/acs.nanolett.7b01254

Ligand Nano-cluster Arrays in a Supported Lipid Bilayer

Emmanuelle Benard, Fuwei Pi, Igor Ozerov, Anne Charrier, Kheya Sengupta

Journal of visualized experiments : JoVE 122:e55060 (2017)10.3791/55060

Tailoring the electrochemical and mechanical properties of PEDOT : pss films for bioelectronics

Mohammed Elmahmoudy, Sahika Inal, Anne Charrier, Ilke Uguz, George Malliaras, Sébastien Sanaur

Macromolecular Materials and Engineering 302 (2017)10.1002/mame.201600497

Plasticity, elasticity, and adhesion energy of plant cell walls: nanometrology of lignin loss using atomic force microscopy

R. Farahi, Anne Charrier, A. Tolbert, A. Lereu, A. Ragauskas, B. Davison, A. Passian

Scientific Reports 7 (2017)10.1038/s41598-017-00234-4

High Aspect Ratio Sub-Micrometer Channels Using Wet Etching: Application to the Dynamics of Red Blood Cell Transiting through Biomimetic Splenic Slits

Priya Gambhire, Scott Atwell, Cécile Iss, Frédéric Bedu, Igor Ozerov, Catherine Badens, Emmanuèle Helfer, Annie Viallat, Anne Charrier

Small 13:1700967 (2017)10.1002/smll.201700967

2016

Subpicomolar Iron Sensing Platform Based on Functional Lipid Monolayer Microarrays

Ahmad Kenaan, Tuyen D. Nguyen, Herve Dallaporta, Jean-Manuel Raimundo, Anne M. Charrier

Analytical Chemistry 88:3804-3809 (2016)10.1021/acs.analchem.5b04834

Shape remodeling and blebbing of active cytoskeletal vesicles

Etienne Loiseau, Jochen A. M. Schneider, Felix C. Keber, Carina Pelzl, Gladys Massiera, Guillaume Salbreux, Andreas R. Bausch

Science Advances 2:UNSP e1500465 (2016)10.1126/sciadv.1500465