{"id":4109,"date":"2019-04-01T11:48:49","date_gmt":"2019-04-01T09:48:49","guid":{"rendered":"http:\/\/www.cinam.univ-mrs.fr\/cinam\/?p=4109"},"modified":"2019-04-01T11:55:22","modified_gmt":"2019-04-01T09:55:22","slug":"blocking-stemness-and-metastatic-properties-of-ovarian-cancer-cells-by-targeting-p70s6k-with-dendrimer-nanovector-based-sirna-delivery","status":"publish","type":"post","link":"https:\/\/www.cinam.univ-mrs.fr\/cinam\/en\/2019\/04\/01\/blocking-stemness-and-metastatic-properties-of-ovarian-cancer-cells-by-targeting-p70s6k-with-dendrimer-nanovector-based-sirna-delivery\/","title":{"rendered":"Blocking stemness and metastatic properties of ovarian cancer cells by targeting p70S6K with dendrimer nanovector-based siRNA delivery"},"content":{"rendered":"<div class=\"groupetitre\">\n<figure id=\"attachment_122\" aria-describedby=\"caption-attachment-122\" style=\"width: 300px\" class=\"wp-caption alignnone\"><img decoding=\"async\" loading=\"lazy\" class=\"size-medium wp-image-122\" src=\"http:\/\/www.cinam.univ-mrs.fr\/cinam\/wp-content\/uploads\/2018\/11\/home-1-300x218.jpg\" alt=\"Ing\u00e9nierie mol\u00e9culaire et mat\u00e9riaux fonctionnels 4\" width=\"300\" height=\"218\" srcset=\"https:\/\/www.cinam.univ-mrs.fr\/cinam\/wp-content\/uploads\/2018\/11\/home-1-300x218.jpg 300w, https:\/\/www.cinam.univ-mrs.fr\/cinam\/wp-content\/uploads\/2018\/11\/home-1-768x557.jpg 768w, https:\/\/www.cinam.univ-mrs.fr\/cinam\/wp-content\/uploads\/2018\/11\/home-1-1024x743.jpg 1024w, https:\/\/www.cinam.univ-mrs.fr\/cinam\/wp-content\/uploads\/2018\/11\/home-1.jpg 1240w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><figcaption id=\"caption-attachment-122\" class=\"wp-caption-text\">Photo : Lorem ipsum dolor sit amet, consectetur adipiscing elit. Donec viverra lectus at posuere fringilla. Suspendisse nec lorem condimentum, sagittis nisi eu.<\/figcaption><\/figure>\n<\/div>\n<p><span class=\"vignette\"><img decoding=\"async\" loading=\"lazy\" class=\"spip_logos\" src=\"http:\/\/sysweb.cinam.univ-mrs.fr\/cinam\/IMG\/arton448.jpg\" alt=\"\" width=\"100\" height=\"129\" \/><\/span><\/p>\n<p class=\"spip\">Jing Ma, Shashwati Kala, Susan Yung, Tak Mao Chan, Yu Cao, Yifan Jiang, Xiaoxuan Liu, Suzanne Giorgio, Ling Peng, and Alice ST Wong<\/p>\n<p class=\"spip\"><strong class=\"spip\"><br \/>\nMol. Ther., 2018, 26, 70-83<\/strong><\/p>\n<p class=\"spip\">Metastasis is the cause of most (&gt;90%) cancer deaths, and currently lack of effective treatments. In this study, we demonstrate the potential advantages of using rationally designed siRNA that targets cancer stem cells (CSCs) in combination with dendrimer nanotechnology-based siRNA delivery for efficacious treatment of metastasis using ovarian cancer as the cancer model. The structurally flexible poly(amidoamine) dendrimer of generation 6 (G<sub>6<\/sub>) formed stable and uniform nanoparticles with p70<sup>S6k<\/sup> siRNA, which protected the siRNA from degradation and significantly decreased the kinase p70<sup>S6K<\/sup> at both mRNA and protein levels in ovarian CSCs, leading to a marked reduction in CSC proliferation and expansion without obvious toxicity towards normal cells. In addition, treatment with the siRNA\/dendrimer nanoparticles substantially decreased mesothelial interaction, migration and invasion of CSCs in vitro as well as tumor growth and metastasis in CSC xenograft mice <i class=\"spip\">in vivo.<\/i> Altogether, these results highlight that the use of siRNA in combination with dendrimer nanotechnology-based delivery constitute a new approach for molecularly targeted cancer therapy to treat metastasis.<br \/>\nThis work is issued from collaboration between Dr. Ling Peng at the CNRS\/Aix-Marseille University in France and Prof. Alice ST Wong at the University of Hong Kong in China.<\/p>\n<p class=\"spip\"><strong class=\"spip\">Contact\u00a0:<\/strong> Ling Peng<\/p>\n<p class=\"spip\"><strong class=\"spip\">Ref\u00a0:<\/strong> Ma et al, Mol. Ther., <strong class=\"spip\">2018<\/strong>, 26, 70-83. (DOI\u00a0: 10.1016\/j.ymthe.2017.11.006<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Jing Ma, Shashwati Kala, Susan Yung, Tak Mao Chan, Yu Cao, Yifan Jiang, Xiaoxuan Liu, Suzanne Giorgio, Ling Peng, and Alice ST Wong Mol. Ther., 2018, 26, 70-83 Metastasis is the cause of most (&gt;90%) cancer deaths, and currently lack of effective treatments. In this study, we demonstrate the potential advantages of using rationally designed <\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[31],"tags":[],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.cinam.univ-mrs.fr\/cinam\/en\/wp-json\/wp\/v2\/posts\/4109"}],"collection":[{"href":"https:\/\/www.cinam.univ-mrs.fr\/cinam\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.cinam.univ-mrs.fr\/cinam\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.cinam.univ-mrs.fr\/cinam\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.cinam.univ-mrs.fr\/cinam\/en\/wp-json\/wp\/v2\/comments?post=4109"}],"version-history":[{"count":1,"href":"https:\/\/www.cinam.univ-mrs.fr\/cinam\/en\/wp-json\/wp\/v2\/posts\/4109\/revisions"}],"predecessor-version":[{"id":4145,"href":"https:\/\/www.cinam.univ-mrs.fr\/cinam\/en\/wp-json\/wp\/v2\/posts\/4109\/revisions\/4145"}],"wp:attachment":[{"href":"https:\/\/www.cinam.univ-mrs.fr\/cinam\/en\/wp-json\/wp\/v2\/media?parent=4109"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.cinam.univ-mrs.fr\/cinam\/en\/wp-json\/wp\/v2\/categories?post=4109"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.cinam.univ-mrs.fr\/cinam\/en\/wp-json\/wp\/v2\/tags?post=4109"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}