{"id":12144,"date":"2023-06-28T09:05:48","date_gmt":"2023-06-28T07:05:48","guid":{"rendered":"https:\/\/www.cinam.univ-mrs.fr\/cinam\/?p=12144"},"modified":"2023-06-28T09:05:48","modified_gmt":"2023-06-28T07:05:48","slug":"soutenance-de-these-26","status":"publish","type":"post","link":"https:\/\/www.cinam.univ-mrs.fr\/cinam\/2023\/06\/28\/soutenance-de-these-26\/","title":{"rendered":"Soutenance de th\u00e8se"},"content":{"rendered":"<p><strong>Titre :<\/strong><br \/>\nMembrane based model systems for integrin mediated adhesion<\/p>\n<p><strong>Abstracts :<\/strong><br \/>\nCells adhere to their surroundings via the cell membrane. When adhering to the extracellular matrix (ECM), transmembrane proteins integrins form mechanosensitive structures (focal adhesions, FAs) that mechanically couple the actomyosin cytoskeleton to the ECM. The long-term objective of our project is to understand the links between actomyosin force, integrin regulation, and ECM properties, which underly FA mechanosensitivity.<br \/>\nWe use a bottom-up approach to reconstitute a minimal adhesion complex on two model membrane systems: synthetic GUVs (Giant Unilamellar Vesicles) and GPMVs (Giant Plasma Membrane Vesicles) derived from cells. We optimized a protocol to make integrin-containing GUVs that were able to adhere to functionalized substrates on one side and to bind integrin regulatory protein talin on the other side, proving the functionality of the embedded integrins. Next, we improved several steps in a protocol to successfully isolate integrin-containing GPMVs from cells.<\/p>\n<p><strong>Jury :<\/strong><br \/>\nMme Stephanie MANGENOT, Universit\u00e9 Paris Cit\u00e9 &#8211; Campus Grands Moulins, Rapporteure<br \/>\nMme Gladys MASSIERA, Universit\u00e9 Montpellier, Rapporteure<br \/>\nM. James STURGIS, Universit\u00e9 Aix-Marseille, Pr\u00e9sident<br \/>\nM. Cl\u00e9ment CAMPILLO, Universit\u00e9 Evry val d&rsquo;essonne, Examinateur<br \/>\nMme Kheya SENGUPTA, Universit\u00e9 Aix-Marseille, Directrice de th\u00e8se<br \/>\nMme Emmanu\u00e8le HELFER, Universit\u00e9 Aix-Marseille, Co-directrice de th\u00e8se<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Mariem Souissi soutiendra le mardi 4 juillet \u00e0 14H00 dans la salle Raymond Kern du CINaM<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[1],"tags":[],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.cinam.univ-mrs.fr\/cinam\/wp-json\/wp\/v2\/posts\/12144"}],"collection":[{"href":"https:\/\/www.cinam.univ-mrs.fr\/cinam\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.cinam.univ-mrs.fr\/cinam\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.cinam.univ-mrs.fr\/cinam\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.cinam.univ-mrs.fr\/cinam\/wp-json\/wp\/v2\/comments?post=12144"}],"version-history":[{"count":3,"href":"https:\/\/www.cinam.univ-mrs.fr\/cinam\/wp-json\/wp\/v2\/posts\/12144\/revisions"}],"predecessor-version":[{"id":12151,"href":"https:\/\/www.cinam.univ-mrs.fr\/cinam\/wp-json\/wp\/v2\/posts\/12144\/revisions\/12151"}],"wp:attachment":[{"href":"https:\/\/www.cinam.univ-mrs.fr\/cinam\/wp-json\/wp\/v2\/media?parent=12144"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.cinam.univ-mrs.fr\/cinam\/wp-json\/wp\/v2\/categories?post=12144"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.cinam.univ-mrs.fr\/cinam\/wp-json\/wp\/v2\/tags?post=12144"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}