Emmanuèle Helfer

Axe

Physique et nano-micro Ingénierie pour le Vivant

E-mail

helfer@cinam.univ-mrs.fr

Phone

+33(0)6 60 30 28 91

Fax

+33(0)4 91 41 89 16

Localisation

TPR1 - 4 étage

Grade

CRCN

Function

chercheur

Room

G4.22

helfer.jpg

Activity

Biomimetism / Membranes / Actin cytoskeleton / Cell Biology / Microscopy / Microfluidics

Themes

Biophysics

Biomimetism, in vitro reconstitution o cellular processes

Membrane Physics

Cell mechanics and dynamics

Recherche

1. Biomimetism: In vitro reconstitution of cellular processes involved in cell membrane organization and remodelling (with Kheya Sengupta)

- control of intracellular traffic

- cell adhesion

Techniques: Optical microscopy (epifluorescence, confocal microscopy, phase contrast, DIC); Micromanipulation (micropipette, optical tweezer); Synthetic membranes (GUVs, supported bilayers); Biochemistry (protein-protein interaction, protein complex purification from mammalian cells); Cell biology (cell culture, immunofluorescence)


2. Cell dynamics and mechanics (with Annie Viallat and Anne Charrier)

- Red Blood Cells (RBCs) in flow (single cell and collective behavior)

- RBCs and cells under mechanical stress (single cell, cell monolayer, cell aggregates)

- Healthy vs pathological cells

Techniques: microfluidics, optical microscopy, micromanipulation

Parcours

Since her PhD, Emmanuèle Helfer was interested in the various interactions between cell membranes and actin, a ubiquitous protein which is strongly involved in mechanical and dynamic properties of cells.
She did her PhD in Strasbourg University with Didier Chatenay and Laurent Bourdieu, working on the viscoelastic properties of actin-coated vesicles mimicking solid shells.
In 2001 she obtained a CNRS position in the 'Cytoskeleton and Cell Motility' group in the Laboratoire d'Enzymologie et Biochimie Structurales (LEBS, Gif-sur-Yvette, France) where she worked on biomimetic reconstitution of actin-based motile processes.
In 2010 she moved to the 'Cytoskeleton and Cell Morphogenesis' group (LEBS) where she studied the role of actin structures on intracellular endosomal membranes by combining cell biology and biochemical approaches.
She joined the new Department 'Physics and micro-nano Engineering of Living Systems' in CINaM in 2014, where she collaborates with other scientists of the Department on membrane-related projects and cell mechanics and goes on developing her biomimetic approach to decipher further the role of endosomal actin.

Publications

2020

Mechanical adaptation of monocytes in model lung capillary networks

Jules Dupire, Pierre-Henri Puech, Emmanuèle Helfer, Annie Viallat

Proceedings of the National Academy of Sciences of the United States of America (2020)

Molecular and Mechanobiological Pathways Related to the Physiopathology of FPLD2

Alice-Anaïs Varlet, Emmanuèle Helfer, Catherine Badens

Cells 9:1947 (2020)10.3390/cells9091947

2019

Self-organization of red blood cell suspensions under confined 2D flows

Cécile Iss, Dorian Midou, Alexis Moreau, Delphine Held, Anne Charrier, Simon Mendez, Annie Viallat, Emmanuèle Helfer

Soft Matter (2019)10.1039/C8SM02571A

2018

When giant vesicles mimic red blood cell's dynamics: swinging of two-phase vesicles in shear flow

Simon Tusch, Etienne Loiseau, Al-Hair Al-Halifa, Kamel Khelloufi, Emmanuèle Helfer, Annie Viallat

Physical Review Fluids 3 (2018)10.1103/PhysRevFluids.3.123605

2017

High Aspect Ratio Sub-Micrometer Channels Using Wet Etching: Application to the Dynamics of Red Blood Cell Transiting through Biomimetic Splenic Slits

Priya Gambhire, Scott Atwell, Cécile Iss, Frédéric Bedu, Igor Ozerov, Catherine Badens, Emmanuèle Helfer, Annie Viallat, Anne Charrier

Small 13:1700967 (2017)10.1002/smll.201700967

2015

Encapsulation of Living Leishmania Promastigotes in Artificial Lipid Vacuoles

Carlos E. S. Guedes, Jose G. B. Lima, Emmanuèle Helfer, Patricia S. T. Veras, Annie Viallat

PLoS ONE 1-12 (2015)10.1371/journal.pone.0134925

Serum Pantetheinase/Vanin Levels Regulate Erythrocyte Homeostasis and Severity of Malaria

Samuel Rommelaere, Virginie Millet, Pascal Rihet, Scott Atwell, Emmanuèle Helfer, Lionel Chasson, Carole Beaumont, Giovanna Chimini, Maria Do Rosario Sambo, Annie Viallat, Carlos Penha-Goncalves, Franck Galland, Philippe Naquet

American Journal of Pathology 185:3039-3052 (2015)10.1016/j.ajpath.2015.07.011

2013

Endosomal recruitment of the WASH complex: Active sequences and mutations impairing interaction with the retromer

Emmanuèle Helfer, Michael Harbour, Véronique Henriot, Goran Lakisic, Carla Sousa-Blin, Larisa Volceanov, Matthew N.J. Seaman, Alexis Gautreau

Biology of the Cell 105:191-207 (2013)10.1111/boc.201200038

2012

Actin Polymerization Controls the Organization of WASH Domains at the Surface of Endosomes

Emmanuel Derivery, Emmanuèle Helfer, Véronique Henriot, Alexis Gautreau

PLoS ONE 7:e39774 (2012)10.1371/journal.pone.0039774

Intermittent depolymerization of actin filaments is caused by photo-induced dimerization of actin protomers

Thomas Niedermayer, Antoine Jégou, Lionel Chièze, Bérengère Guichard, Emmanuèle Helfer, Guillaume Romet-Lemonne, Marie-France Carlier, Reinhard Lipowsky

Proceedings of the National Academy of Sciences of the United States of America 109:10769-10774 (2012)10.1073/pnas.1121381109

2011

Force-Velocity Measurements of a Few Growing Actin Filaments

Coraline Brangbour, Olivia Du Roure, Emmanuèle Helfer, Damien Démoulin, Alexis Mazurier, Marc Fermigier, Marie-France Carlier, Jérôme Bibette, Jean Baudry

PLoS Biology 9:e1000613 (2011)10.1371/journal.pbio.1000613

BIOMIMETIC SYSTEMS SHED LIGHT ON ACTIN-BASED MOTILITY DOWN TO THE MOLECULAR SCALE

Guillaume Romet-Lemonne, Emmanuèle Helfer, Vincent Delatour, Beata Bugyi, Montserrat Bosch, Stephane Romero, Marie-France Carlier, Stephan Schmidt, Andreas Fery

Biophysical Reviews and Letters 04:5-15 (2011)10.1142/S1793048009000909

2010

Force Generation of Curved Actin Gels Characterized by Combined AFM-Epifluorescence Measurements

Stephan Schmidt, Emmanuèle Helfer, Marie-France Carlier, Andreas Fery

Biophysical Journal 98:2246-2253 (2010)10.1016/j.bpj.2010.01.055

2008

Arp2/3 Controls the Motile Behavior of N-WASP-Functionalized GUVs and Modulates N-WASP Surface Distribution by Mediating Transient Links with Actin Filaments

Vincent Delatour, Emmanuèle Helfer, Dominique Didry, Kim Ho Diep Le, Jean-François Gaucher, Marie-France Carlier, Guillaume Romet-Lemonne

Biophysical Journal 94:4890-4905 (2008)10.1529/biophysj.107.118653

2007

Adapter Protein SH2-B Stimulates Actin-Based Motility of Listeria monocytogenes in a Vasodilator-Stimulated Phosphoprotein (VASP)-Dependent Fashion

Maria Diakonova, Emmanuèle Helfer, Stéphanie Seveau, Joel A. Swanson, Christine Kocks, Liangyou Rui, Marie-France Carlier, Christin Carter-Su

Infection and Immunity 75:3581-3593 (2007)10.1128/iai.00214-07

Structural basis and evolutionary origin of actin filament capping by twinfilin

Ville O Paavilainen, Maarit Hellman, Emmanuèle Helfer, Miia Bovellan, Arto Annila, Marie-France Carlier, Perttu Permi, Pekka Lappalainen

Proceedings of the National Academy of Sciences of the United States of America 104:3113-3118 (2007)10.1073/pnas.0608725104

2006

Mammalian twinfilin sequesters ADP-G-actin and caps filament barbed ends: implications in motility

Emmanuèle Helfer, Elisa Nevalainen, Perttu Naumanen, Stéphane Romero, Dominique Didry, Dominique Pantaloni, Pekka Lappalainen, Marie-France Carlier

EMBO Journal 25:1184-1195 (2006)10.1038/sj.emboj.7601019

2005

The Interplay between Viscoelastic and Thermodynamic Properties Determines the Birefringence of F-Actin Gels

Emmanuèle Helfer, Pierre Panine, Marie-France Carlier, Patrick Davidson

Biophysical Journal 89:543-553 (2005)10.1529/biophysj.104.050245

2003

A biomimetic motility assay provides insight into the mechanism of actin-based motility

Sebastian Wiesner, Emmanuèle Helfer, Dominique Didry, Guylaine Ducouret, Françoise Lafuma, Marie-France Carlier, Dominique Pantaloni

Journal of Cell Biology 160:387-398 (2003)10.1083/jcb.200207148

2001

Buckling of Actin-Coated Membranes under Application of a Local Force

E. Helfer, S. Harlepp, L. Bourdieu, J. Robert, F. Mackintosh, D. Chatenay

Physical Review Letters 87:088103 (2001)

Viscoelastic properties of actin-coated membranes

E. Helfer, S. Harlepp, L. Bourdieu, J. Robert, F. Mackintosh, D. Chatenay

Physical Review E 63:021904 (2001)

2000

Microrheology of Biopolymer-Membrane Complexes

E. Helfer, S. Harlepp, L. Bourdieu, J Robert, F Mackintosh, D. Chatenay

Physical Review Letters 85:457-60 (2000)