Kheya Sengupta

Localisation

TPR1 - 4 étage

Grade

DR1

Fonction

chercheur

Pièce

G.04.20

sengupta.jpg

Activity

Soft-matter, Biophysics, Nano-science

Thèmes

ResearcherID: E-8629-2011 || ORCID: 0000-0002-1060-271

Brief CV: Kheya Sengupta did her PhD on liquid-crystalline phases of phospholipids at RRI, Bangalore with V. A, Raghunathan. She moved to Munich as a post-doctoral fellow with an Alexander von Humboldt fellowship, to study physics of bio-membranes and bio-polymers at the laboratory of Erich Sackmann. Later, she went on to study cell mechanics and cell adhesion in Paul Janmey’s lab at U-Penn, Philadelphia. She came back to Germany as a research associate at the Forschungszentrum Julich in the laboratory of Rudolf Merkel, where she continued her work on adhesion, including further development of reflection interference contrast microscopy. In 2007 she joined the National Scientific Research Council of France (CNRS) with a permanent position as independent researcher and moved to the interdisciplinary unit of CINaM in Marseilles, where she develops novel optical techniques and surface nano-patterning tools to study adhesion and mechanical properties of cells and cell mimetic model systems. Her research in the last decade has revealed the physical basis of entropy dominated membrane adhesion, and the role of membrane fluctuations. In recent years, she has contributed to the understanding of the response of T cells to environmental mechanics, notably shedding light on TCR-coupling to the cytoskeleton. Her overall research interest encompasses fundamental questions ranging from model membranes to immune therapy.

 

Current research:

  • Adhesion and fluctuations of model membranes.
  • Adhesion, mechanics and activation of T lymphocytes.
  • Development of novel surface nano-patterning techniques for controlled stimulation of cells.
  • Development of novel optical imaging tools for imaging cell/membrane adhesion and fluctuations.

Some of the completed work is summarized in the following book chapters and reviews:

Reviews:

·       Quantifying dynamical adhesion processes with Reflection interference contrast microscopy (RICM), Laurent Limozin and Kheya Sengupta. Invited review ChemPhysChem, 10(16):2752-2768 (2009).

·       Giant Vesicles as Cell Models. Susanne Fenz and Kheya Sengupta, Integrative Biology, 4 (2012) 982–995.

·       Shape Fluctuations in Biological Membranes - Experimental Techniques and Biophysical Significance, Cornelia Monzel and Kheya Sengupta. Invited Review JPhys. D. 29 (24), 243002 (2016) .

·       Kheya Sengupta, Pierre Dillard, Laurent Limozin, Morphodynamics of T-lymphocytes: Scanning to spreading, BiophysJ.2024 10.1016/j.bpj.2024.02.023.

Book chapters:

·       Membrane Adhesion, Kheya Sengupta and Ana-Suncana Smith, in "Physics of Biological Membranes", Edited by Patricia Bassereau and Pierre Sens. Springer (2018) ISBN 978-3-030-00630-3 (https://www.springer.com/gp/book/9783030006280).

·       Measuring Giant Vesicle adhesion, Ana-Suncana Smith and Kheya Sengupta, in "The Giant Vesicle Book", Edited by Carlos Marquez and Rumiana Dimova, CRC Press (2019) ISBN 9781315152516.

·       Observing Membrane and Cell Adhesion via Reflection Interference Contrast Microscopy., Ahmed Abdelrahman, Ana-Suncana Smith and Kheya Sengupta, in Springer Protocols Methods in molecular biology, The Immune Synapse Methods and Protocols 2654 (2023): 123-135.

Parcours

Research positions:

·       January 2007-present: Scientific researcher, CINaM-CNRS.

·       April 2005 – December 2006: Staff Scientist, Institut für Bio- und Nanosysteme (IBN-4), Biomechanik, Forschungszentrum Jülich GmbH, Leo-Brandt-Straße, 52428 Jülich, Deutschland.

·       February 2004 - March 2005: Post-doctoral research fellow, Institute of Medicine and Engineering, School of Medicine, University of Pennsylvania, 1010 Vagelos Labs., 3340 Smith Walk, Philadelphia, PA 19104 , USA.

·       March 2001 - December 2003: Post-doctoral research fellow. (March 2001 – November 2002: Alexander von Humboldt fellow, November 2002- August 2003: Fellow of the Max Plank Institute),Physik-Department - E22 (Biophysik), Technische Universität München, James-Franck-Strasse, D-85748 Garching, Deutschland.

·       August 2000 - February 2001: Research fellow, Department of Physics, Indian Institute of Science, Bangalore 560012, India.

·       August 1995 - July 2000: Ph.D. in Physics Raman Research Institute, Bangalore, India. Thesis: Investigations on the ripple phase of phospholipid bilayers (Advisor: V. A. Raghunathan

Research Publications (from 2015 onwards):

·       Crowding of receptors induces ring-like adhesions in model membranes, D. Schmidt, T. Bihr, S. Fenz, R. Merkel, U. Seifert, K. Sengupta, and A.-S. Smith. Biochimica Et Biophysica Acta-Molecular Cell Research 1853 (11) 2984-2991 (2015).

·       Size-Tunable Organic Nanodot Arrays: A Versatile Platform for Manipulating and Imaging Cells, Fuwei Pi, Pierre Dillard, Ranime Alameddine, Emmanuelle Benard, Astrid Wahl, Igor Ozerov, Anne Charrier, Laurent Limozin, and Kheya Sengupta, Nano Letters, 15 (8) 5178-5184 (2015).

·       Measuring fast stochastic displacements of bio-membranes with dynamic optical displacement spectroscopy.  C. Monzel, D. Schmidt, C. Kleusch, D. Kirchenbuechler, U. Seifert, A.-S. Smith, K. Sengupta and R. Merkel. Nature communications. 6, 8162 (2015).

·       Nano-clustering of Ligands on Surrogate Antigen Presenting Cells Modulates T cell Membrane Adhesion and Organization. Pierre Dillard, Fuwei Pi, Annemarie Lellouche, Laurent Limozin, Kheya Sengupta. Integrative Biology. 8 (3), 287-301 (2016).

·       Nanometric Thermal Fluctuations of Weakly Confined Biomembranes Measured with Microsecond Time-Resolution, Cornelia Monzel, Daniel Schmidt, Udo Seifert, Ana-Suncana Smith, Rudolf Merkel, and Kheya Sengupta. SoftMatter 12, 4755-4768 (2016).

·       Protein nano-dots in supported lipid bilayers, E. Benard, F. Pi, I. Ozerov, A. Charrier and K. Sengupta, JOVE, accepted (2017).

·       Membrane fluctuations mediate lateral interaction between cadherin bonds, Susanne Fenz, Timo Bihr, Daniel Schmidt, Rudolf Merkel, Udo Seifert, Kheya Sengupta, and Ana-Suncana Smith. Nature Physics, 13, 906–913 (2017).

·       Printing functional protein nano-dots on soft elastomers: from transfer mechanism to cell mechanosensing, Ranime Alameddine, Astrid Wahl, Fuwei Pi, Kaoutar Bouzalmate, Laurent Limozin, Anne Charrier, and Kheya Sengupta. Nano Lett., 2017, 17 (7): 4284–4290.

·       T cells on engineered substrates: the impact of TCR clustering is enhanced by LFA-1 engagement, Emmanuelle Benard, Jacques A. Nunes, Laurent Limozin and Kheya Sengupta. Front. Immunol. 2018, doi: 10.3389/fimmu.2018.02085.

·       Lamellipod Reconstruction by Three-Dimensional Reflection Interference Contrast Nanoscopy (3D-RICN). M-J. Dejardin, A. Hemmerle, A. Sadoun, Y. Hamon, P-H. Puech, K. Sengupta, and L. Limozin Nano Lett., 2018 (DOI: 10.1021/acs.nanolett.8b03134).

·       Biphasic mechanosensitivity of TCR mediated adhesion of T lymphocytes. A. Wahl, C. Dinet, P. Dillard, P-H. Puech, L. Limozin, and K. Sengupta. PNAS (2019) 116 (13) 5908-5913.

·       Ligand Nanocluster Array Enables Artificial-Intelligence-Based Detection of Hidden Features in T-Cell Architecture, Nano Letters (2021). Nano Lett. 21 (13) 5606–5613 DOI: 10.1021/acs.nanolett.1c01073.

·       Integrin-Functionalised Giant Unilamellar Vesicles via Gel-Assisted Formation: Good Practices and Pitfalls, International Journal of Molecular Sciences (2021) DOI: 10.3390/ijms22126335.

·       On the control of dispersion interactions between biological membranes and protein coated biointerfaces, Blackwell, Robert; Hemmerle, Arnaud; Baer, Andreas; Sengupta, Kheya; Smith, Ana-Suncana. Journal of Colloid and Interface Science (2021) DOI: 10.1016/J.JCIS.2021.02.078

·       Physics of Organelle Membrane Bridging via Cytosolic Tethers is Distinct From Cell Adhesion ; Kamal Mohammad Arif, Janeš Josip Augustin, Li Long, Thibaudau Franck, Smith Ana-Sunčana, Sengupta Kheya, Frontiers in Physics 9 (2022).

·       Biomechanics as driver of aggregation of tethers in adherent membranes, Long Li, Mohammad Arif Kamal, Bernd Henning Stumpf, Franck Thibaudau, Kheya Sengupta and Ana-Sunčana Smith.Soft Matter,17, 10101-10107 (2021).

·       May the force be with your (immune) cells: an introduction to traction force microscopy in Immunology. F. Mustapha, K. Sengupta, P-H. Puech.  Frontiers in Immunology (2022). DOI: 10.3389/fimmu.2022.898558.

·       Protocol for measuring weak cellular traction forces using well-controlled ultra-soft polyacrylamide gels, F. Mustapha, K. Sengupta, P-H. Puech. STAR Protocols (2022) DOI: 10.1016/j.xpro.2022.101133.

·       Physics of Organelle Membrane Bridging via Cytosolic Tethers is Distinct From Cell Adhesion Frontiers in Physics (2022) DOI: 10.3389/fphy.2021.750539.

·       First-Principle Coarse-Graining Framework for Scale-Free Bell-Like Association and Dissociation Rates in Thermal and Active Systems, J. A. Janes, C. Monzel, D. Schmidt, R. Merkel, Rudolf, U. Seifert, K. Sengupta, A-S. Smith. Phys. Rev. X (2022) 12 (3) 031030. https://link.aps.org/doi/10.1103/PhysRevX.12.031030.

·       Manca, F., Eich, G., N’Dao, O. et al. Probing mechanical interaction of immune receptors and cytoskeleton by membrane nanotube extraction. Sci Rep 13, 15652 (2023). https://doi.org/10.1038/s41598-023-42599-9.

·       Sengupta K., Dillard P., Limozin L., Morphodynamics of T-lymphocytes: Scanning to spreading, BiophysJ.2024 10.1016/j.bpj.2024.02.023

Patent: Nano−scale stimulating substrates, Sengupta, Benard, Ozrov, Bedu, Dallaporta, FR1853414 2018, WO/2019/202260. 2019.

Publications

2023

Probing mechanical interaction of immune receptors and cytoskeleton by membrane nanotube extraction

Fabio Manca, Gautier Eich, Omar N’dao, Lucie Normand, Kheya Sengupta, Laurent Limozin, Pierre-Henri Puech

Scientific Reports 13:15652 (2023)10.1101/2022.09.15.508080

Talin and kindlin cooperate to control the density of integrin clusters

Julien Pernier, Marcelina Cardoso Dos Santos, Mariem Souissi, Adrien Joly, Hemalatha Narassimprakash, Olivier Rossier, Grégory Giannone, Emmanuèle Helfer, Kheya Sengupta, Christophe Le Clainche

Journal of Cell Science 136:jcs260746 (2023)10.1242/jcs.260746

2022

First-Principle Coarse-Graining Framework for Scale-Free Bell-Like Association and Dissociation Rates in Thermal and Active Systems

Josip Augustin Janeš, Cornelia Monzel, Daniel Schmidt, Rudolf Merkel, Udo Seifert, Kheya Sengupta, Ana-Sunčana Smith

Physical Review X 12:031030 (2022)10.1103/PhysRevX.12.031030

Physics of Organelle Membrane Bridging via Cytosolic Tethers is Distinct From Cell Adhesion

Mohammad Arif Kamal, Josip Augustin Janeš, Long Li, Franck Thibaudau, Ana-Sunčana Smith, Kheya Sengupta

Frontiers in Physics 9:750539 (2022)10.3389/fphy.2021.750539

May the force be with your (immune) cells: an introduction to traction force microscopy in Immunology

Farah Mustapha, Kheya Sengupta, Pierre-Henri Puech

Frontiers in Immunology 13:898558 (2022)10.3389/fimmu.2022.898558

2021

On the control of dispersion interactions between biological membranes and protein coated biointerfaces

Robert Blackwell, Arnaud Hemmerle, Andreas Baer, Matthias Späth, Wolfgang Peukert, Drew Parsons, Kheya Sengupta, Ana-Sunčana Smith

Journal of Colloid and Interface Science 598:464-473 (2021)10.1016/j.jcis.2021.02.078

Biomechanics as driver of aggregation of tethers in adherent membranes

Long Li, Mohammad Arif Kamal, Bernd Henning Stumpf, Franck Thibaudau, Kheya Sengupta, Ana-Sunčana Smith

Soft Matter 17:10101-10107 (2021)10.1039/d1sm00921d

Ligand Nanocluster Array Enables Artificial-Intelligence-Based Detection of Hidden Features in T-Cell Architecture

Aya Nassereddine, Ahmed Abdelrahman, Emmanuelle Benard, F. Bedu, Igor Ozerov, Laurent Limozin, Kheya Sengupta

Nano Letters 21:5606-5613 (2021)10.1021/acs.nanolett.1c01073

On the control of dispersion interactions between biological membranes and protein coated biointerfaces

Kheya Sengupta, Robert Blackwell, Arnaud Hemmerle, Andreas Baer, Matthias Späth, Wolfgang Peukert, Drew Parsons, Ana-Sunčana Smith

Journal of Colloid and Interface Science 598:464-473 (2021)10.1016/j.jcis.2021.02.078

2019

Biphasic mechanosensitivity of T cell receptor-mediated spreading of lymphocytes

Astrid Wahl, Céline Dinet, Pierre Dillard, Aya Nassereddine, Pierre-Henri Puech, Laurent Limozin, Kheya Sengupta

Proceedings of the National Academy of Sciences of the United States of America 116:5908-5913 (2019)10.1073/pnas.1811516116

2018

T Cells on Engineered Substrates: The Impact of TCR Clustering Is Enhanced by LFA-1 Engagement

Emmanuelle Benard, Jacques Nunès, Laurent Limozin, Kheya Sengupta

Frontiers in Immunology 9 (2018)10.3389/fimmu.2018.02085

Lamellipod Reconstruction by Three-Dimensional Reflection Interference Contrast Nanoscopy (3D-RICN)

Marie-Julie Dejardin, Arnaud Hemmerle, Anais Sadoun, Yannick Y. Hamon, Puech Pierre-Henri, Kheya Sengupta, Laurent Limozin

Nano Letters 18:6544-6550 (2018)10.1021/acs.nanolett.8b03134

2017

Printing functional protein nano-dots on soft elastomers: from transfer mechanism to cell mechanosensing

Ranime Alameddine, Astrid Wahl, Fuwei Pi, Kaoutar Bouzalmate, Laurent Limozin, Anne Charrier, Kheya Sengupta

Nano Letters 17:4284-4290 (2017)10.1021/acs.nanolett.7b01254

Ligand Nano-cluster Arrays in a Supported Lipid Bilayer

Emmanuelle Benard, Fuwei Pi, Igor Ozerov, Anne Charrier, Kheya Sengupta

Journal of visualized experiments : JoVE 122:e55060 (2017)10.3791/55060

Membrane fluctuations mediate lateral interaction between cadherin bonds

Susanne Fenz, Timo Bihr, Daniel Schmidt, Rudolf Merkel, Udo Seifert, Kheya Sengupta, Ana-Sunčana Smith

Nature Physics 13:906-913 (2017)10.1038/NPHYS4138

2016

Nano-clustering of ligands on surrogate antigen presenting cells modulates T cell membrane adhesion and organization

Pierre Dillard, Fuwei Pi, Annemarie Lellouch, Laurent Limozin, Kheya Sengupta

Integrative biology 8:287-301 (2016)10.1039/C5IB00293A

Membrane Mediated Cooperativity Facilitates Cadherin Clustering in Model Membranes

Susanne Fenz, Timo Bihr, Daniel Schmidt, Rudolf Merkel, Kheya Sengupta, Udo Seifert, Ana-Suncana Smith

Biophysical Journal 110:190A (2016)10.1016/j.bpj.2015.11.1058

Measuring shape fluctuations in biological membranes

C. Monzel, K. Sengupta

Journal of Physics D: Applied Physics 49:243002 (2016)10.1088/0022-3727/49/24/243002

Dynamic Optical Displacement Spectroscopy to Quantify Biomembrane Bending Fluctuations

Cornelia Monzel, Daniel Schmidt, Udo Seifert, Ana-Suncana Smith, Kheya Sengupta, Rudolf Merkel

Biophysical Journal 110:487A (2016)10.1016/j.bpj.2015.11.2603

Nanometric thermal fluctuations of weakly confined biomembranes measured with microsecond time-resolution

Cornelia Monzel, Daniel Schmidt, Udo Seifert, Ana-Suncana Smith, Rudolf Merkel, Kheya Sengupta

Soft Matter 12:4755-4768 (2016)10.1039/c6sm00412a

2015

Size-Tunable Organic Nanodot Arrays: A Versatile Platform for Manipulating and Imaging Cells

Fuwei Pi, Pierre Dillard, Ranime Alameddine, Emmanuelle Benard, Astrid Wahl, Igor Ozerov, Anne Charrier, Laurent Limozin, Kheya Sengupta

Nano Letters 15:5178-5184 (2015)10.1021/acs.nanolett.5b01400

2014

Signature of a Nonharmonic Potential as Revealed from a Consistent Shape and Fluctuation Analysis of an Adherent Membrane

D. Schmidt, C. Monzel, T. Bihr, R. Merkel, U. Seifert, K. Sengupta, A.S. Smith

Physical Review X 4:021023 (2014)10.1103/PhysRevX.4.021023

2013

Nanometric Protein-Patch Arrays on Glass and Polydimethylsiloxane for Cell Adhesion Studies

Fuwei Pi, Pierre Dillard, Laurent Limozin, Anne Charrier, Kheya Sengupta

Nano Letters 13:3372-3378 (2013)10.1021/nl401696m

A bola-phospholipid containing tetrafluorophenylazido chromophore as a promising lipid probe for biomembrane photolabeling studies

Y. Xia, K. Sengupta, A. Maggiani, F. Qu, Ling Peng

Organic & Biomolecular Chemistry 11:5000-5005 (2013)

2012

Giant Vesicles as Cell Models

S.F. Fenz, K. Sengupta

Integrative Biology 4:982-995 (2012)

Mapping Fluctuations in Biomembranes Adhered to Micropatterns

C. Monzel, S.F. Fenz, M. Giesen, R. Merkel, K. Sengupta

Soft Matter 8:6128-6138 (2012)

Heavy fermion superconductor CeCu 2 Si 2 under high pressure: Multiprobing the valence crossover

Gabriel Seyfarth, A.-S. Rüetschi, K. Sengupta, A. Georges, D. Jaccard, S. Watanabe, K. Miyake

Physical Review B: Condensed Matter and Materials Physics (1998-2015) 85:205105 (2012)10.1103/PhysRevB.85.205105

Proximity to valence transition in heavy fermion superconductor CeCu 2 Si 2 under pressure

Gabriel Seyfarth, A.-S. Rüetschi, K. Sengupta, A. Georges, D. Jaccard

EPL - Europhysics Letters 98:17012 (2012)10.1209/0295-5075/98/17012

Depth matters: cells grown on nano-porous anodic alumina respond to pore depth

S. Thakur, S. Massou, A.M. Benoliel, P. Bongrand, M. Hanbücken, K. Sengupta

Nanotechnology 23:255101 (2012)