Kheya Sengupta

Activité

Soft-matter, Biophysics, Nano-science

Thèmes

ResearcherID: E-8629-2011 || ORCID: 0000-0002-1060-2713

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My current research is focused on adhesion of living and model cells. The adhesion of cells is a fundamental biological process with implications for functions ranging from tissue morphogenesis to immune response. Very often, the primary focus in cell adhesion research is on identifying the relevant adhesion proteins and signaling pathways. However, for a complete description, it is essential to also understand the physics underlying the processes that govern the adhesion. In order to study a complex phenomenon like cell adhesion, in-vitro experiments using cell mimetic systems are often useful since the experimental conditions can be better controlled. Moreover, the simplicity of such systems facilitates theoretical modeling. The insight gained from such bio-mimetic studies can then be applied to real cells. In my research, on one hand I try to make realistic models of adhering cells using giant unilamellar vesicles and on the other hand I study the adhesion of real cells under biomimetic conditions to assess the relevance of the vesicle models.

Recherche

Current research interests:

1. Nano-patterning for cell studies

2. Adhesion and fluctuations of membranes

3. Quantitative optical techniques for cell adhesion

4. Adhesion and mechanics of T lymphocytes

Collaborations:

Ana Smith, FAU, Germany.

Laurent Limozin, CNRS, France.

Pierre-Henri Pueche, INSERM, France.

Christophe LeClainche, CNRS, France.

Funding past and present:

ERC 2013-2017

ANR 2018-2022

CNRS 2007-

AMIDEX 2014-2017

Region PACA 2009-2010

Parcours

Curriculum Vitae: Kheya Sengupta did her PhD in Bangalore with V. A, Raghunathan on liquid-crystalline phases of phospholipids. In 2001 she moved to the laboratory of Erich Sackmann in Munich with an Alexander von Humboldt fellowship. She studied bio-mimetic systems and contributed to the development of multi-wavelength RIC microscopy. In 2004 she moved to University of Pennsylvania, where she worked with Paul Janmey on cell mechanics and cell adhesion. In 2005 she came back to Germany as a research associate at the Forschungszentrum Julich in the laboratory of Rudolf Merkel, where she continued her work on adhesion and continued to improve RIC microscopy. In 2007 she was offered the position of a researcher of the National Scientific Research Council of France (CNRS) and moved to Marseilles. Her current interests include adhesion and mechanical properties of cells and cell mimetic model systems, and the interaction of such soft systems with nanoscale objects.

Positions: January 2007-present: Scientific researcher, CINaM-CNRS. April 2005 – December 2006: Staff Scientist, Institut für Bio- und Nanosysteme (IBN-4), Biomechanik, Forschungszentrum Jülich GmbH, Leo-Brandt-Straße, 52428 Jülich, Deutschland. February 2004 - March 2005: Post-doctoral research fellow, Institute of Medicine and Engineering, School of Medicine, University of Pennsylvania, 1010 Vagelos Labs., 3340 Smith Walk, Philadelphia, PA 19104 , USA. March 2001 - December 2003: Post-doctoral research fellow. (March 2001 – November 2002: Alexander von Humboldt fellow, November 2002- August 2003: Fellow of the Max Plank Institute),Physik-Department - E22 (Biophysik), Technische Universität München, James-Franck-Strasse, D-85748 Garching, Deutschland. August 2000 - February 2001: Research fellow, Department of Physics, Indian Institute of Science, Bangalore 560012, India.

Publications:

1. The structure of the ripple phase in chiral and racemic DMPC multibilayers., Kheya Sengupta, V. A. Raghunathan and John Katsaras, Phys. Rev. E 59 (2), 2455 (1999).

2. Fascinating Shapes and Structures Due to Entropic Forces, Resonance, Kheya Sengupta and Kattera A Suresh, Resonance 1 (4), 68 (1999).

3. Novel structural features of the ripple phase of phospholipids., Kheya Sengupta, V. A. Raghunathan and John Katsaras, Euro. Phys. Lett. 49, 722 (2000).

4. The role of tilt order in the asymmetric ripple phase of phospholipid bilayers., Kheya Sengupta, V. A. Raghunathan and Yashodhan Hatwalne, Phys. Rev. Lett. 87, 055705 (2001).

5. Structure of the ripple phase in phospholipids., Kheya Sengupta, V. A. Raghunathan and John Katsaras, Phys. Rev. E 68, 031710 (2003).

6. Mimicking tissue surfaces by supported membrane coupled ultra-thin layer of hyaluronic acid., Kheya Sengupta, Jörg Schilling, Stefanie Marx, Markus Fischer, Adelbert Bacher and Erich Sackmann, Langmuir 19 (5), 1775 (2003).

7. Absolute Interfacial Distance Measurements by Dual Wavelength Reflection Interference Contrast Microscopy., Jörg Schilling, Kheya Sengupta, Stefanie Gönnenwein, Andeas Bausch, and Erich Sackmann., Phys. Rev. E 69, 021901 (2004).

8. Probing multilayers of polyelectrolytes by Dual wavelength reflection interference contrast microscopy., Catherine Picart, Kheya Sengupta, Jörg Schilling, Gjertrud Maurstad, Guy Ladam, Andreas Bausch and Erich Sackmann. J. Phys. Chem. B 180 (22) 7196 (2004).

9. Topographical pattern dynamics in passive adhesion of cell membranes, A. Popescu Hategan, Kheya Sengupta, Samuel Kahn, Erich Sackmann, Dennis Discher. Biophys. J. 87, 3547 (2004).

10. Frequency dependent shape changes in electric field induced 2-d colloidal clusters., Ajay Singh Negi, Kheya Sengupta and Ajay K. Sood, Langmuir 21, 11623 (2005).

11. Coupling of artificial actin cortices to bio-functionalised lipid monolayers., Kheya Sengupta, Laurent Limozin, Matthias Tristl, Ilka Haase, Markus Fischer and Erich Sackmann. Langmuir 22, 5776 (2006).

12. Adhesion of neutrophils: from activation to migration., Kheya Sengupta, Helim Aranda-Espinoza, Lee Smith, Paul Janmey and Daniel Hammer. Biophys. J. 91, 4638 (2006).

13. Modulation of vesicle adhesion and spreading kinetics by hyaluronan cushions, Laurent Limozin and Kheya Sengupta. Biophys J. 93:3300-13 (2007).

14. Fibroblast adaptation and stiffness matching to soft elastic substrates , Jerome Solon, Ilya Levental, Kheya Sengupta, Penelope C Georges, and Paul A Janmey. Biophys J. 93:4453-61 (2007).

15. Measuring mechanical properties of polyelectrolyte multilayer thin films: Novel methods based on AFM and optical techniques, Catherine Picart, Bernard Senger, Kheya Sengupta, Frederic Dubreuil, Andreas Fery, colloids. & surfaces A. 303: 30-36 (2007).

16. Force-induced growth of adhesion domains is controlled by receptor mobilit, Ana-Sunčana Smith, Kheya Sengupta, Stefanie Gönnenwein, Udo Seifert and Erich Sackmann. Proc. Nat. Acad. Sc. USA 105:6906-11 (2008).

17. Tuning the formation and rupture of single ligand-receptor bonds by hyaluronan-induced repulsion, Philippe Robert, Kheya Sengupta, Pierre-Henri Puech, Pierre Bongrand, and Laurent Limozin. Biophys J 95:3999-4012 (2008).

18. Dynamics of specific vesicle-substrate adhesion: From local events to global dynamics. Ellen Reister-Gottfried, Kheya Sengupta, Barbara Lorz, Erich Sackmann, Udo Seifert, and Ana-Suncana Smith. Phys. Rev. Lett. 101:208103 (2008).

19. Diffusion and inter-membrane distance: a case study of avidin and E-cadherin mediated adhesion, Susanne Fenz, Rudolf Merkel and Kheya Sengupta., Langmuir 25:1074 (2009).

20. Large scale ordered plastic nano-pillars for quantitative live-cell imaging. Kheya Sengupta, Eric Moyen, Magali Mace, Anne-Marie Benoliel, Anne Pierres, Frank Thibaudau, Laurence Masson, Laurent Limozin, Pierre Bongrand, and Margrit Hanbücken. Small, 5:449 (2009).

21. Large scale ordered topographical and chemical nano-features from anodic alumina templates, Sophie Massou, Laurence Masson, Igor Ozerov, Eric Moyen and Kheya Sengupta and Margrit Hanbücken, Ap. Su. Sc., 256:395-398 (2009).

22. Selective functionalization of substrates through assembled nanostructures: From physics to biology, Eric Moyen, Magali Macé, Laurence Masson, Kheya Sengupta and Margrit Hanbücken, Ap. Su. Sc. 256:414-418 (2009).

23. Quantifying dynamical adhesion processes with Reflection interference contrast microscopy (RICM), Laurent Limozin and Kheya Sengupta. Invited review ChemPhysChem, 10(16):2752-2768 (2009).

24. Probing Bio–Membrane Dynamics by Dual–Wavelength Reflection Interference Contrast Microscopy, Cornelia Monzel, Susanne Fenz, Rudolf Merkel, and Kheya Sengupta. ChemPhysChem, 10(16):2828-2838 (2009).

25. Determination of nano-ordering within adhesion clusters: fluctuations as a contrast-enhancement tool, Ana Smith, Susanne Fenz, and Kheya Sengupta, Euro. Phys. Lett. 89(2): 28003-28009 (2010).

26. Adhesion of soft membranes controlled by tension and interfacial polymers, Kheya Sengupta and Laurent Limozin. Phys. Rev. Lett. 104(8), 088101-088105 (2010).

27. Blebbing as a means of spreading: RICM studies on endothelial cells, Lean Matta, Jan Brugués, Kheya Sengupta, Pierre Sens and Helim Aranda-Espinoza. BiophysJ. 99(6), 1726-1730 (2010).

28.Novel anodic aluminum oxide-based nanofabrication: applications in physics and biology, Moyen E, Sahaf H, Mace M, L. Masson, K.Sengupta, M. Hanbucken. Surf. Interf. Anal. 42(10) 1556-1560 (2010).

29.Inter-membrane Adhesion Mediated by Mobile Linkers: Effect of Receptor Shortage, S. Fenz, A-S Smith, R. Merkel and K. Sengupta. Soft Matter 7, 952-962 (2011). (Selected as "Hot-Article")

30.Blebbing dynamics during endothelial cell spreading, L. Matta, K. Sengupta, and H. Aranda-Espinoza. Eur. J. Cell Biol. 90(1):37-48 (2011).

31. Switching from ultra-weak to strong adhesion, Susanne Franziska Fenz, Timo Bihr, Rudolf Merkel, Udo Seifert, Kheya Sengupta, Ana-Sunčana Smith. Adv. Materials. 23 (22-23):2622-2626 (2011).

32. Photoactivatable phospholipids bearing tetrafluorophenylazido chromophores exhibit unprecedented protonation-state-dependent (19)f NMR signals. Yi Xia, Fanqi Qu, Alain Maggiani, Kheya Sengupta, Cheng Liu, Ling Peng. Org. lett. 13 (16):4248-51 (2011).

33. Mapping Fluctuations in Biomembranes Adhered to Micropatterns. Cornelia Monzel, Susanne F. Fenz, Margret Giesen, Rudolf Merkel, and Kheya Sengupta, Soft Matter, 8 (2012) 6128-6138.

34. Depth matters: cells grown on nano-porous anodic alumina respond to pore depth. Shreyasi Thakur, Sophie Massou, Anne-Marie Benoliel, Pierre Bongrand, Margrit Hanbucken and Kheya Sengupta. Nanotechnology, 23 (2012) 255101. (Editor's Choice)

35. Giant Vesicles as Cell Models. Susanne F. Fenz and Kheya Sengupta, Integrative Biology, 4 (2012) 982–995.

36. A bola-phospholipid containing tetrafluorophenylazido chromophore as a promising lipid probe for biomembrane photolabeling studies Y. Xia, K. Sengupta, A. Maggiani, F. Qu, L. Peng, Organic & Biomolecular Chemistry / Organic and Biomolecular Chemistry 11 5000-5005 (2013).

37. Nanometric Protein-Patch Arrays on Glass and Polydimethylsiloxane for Cell Adhesion Studies, F. Pi, P. Dillard, L. Limouzin, A. Charrier, K. Sengupta, Nano Letters 13 7 3372-3378 (2013).

38. Signature of a non-harmonic potential as revealed from a consistent shape and fluctuation analysis of an adherent membrane, Daniel Schmidt, Cornelia Monzel, Timo Bihr, Rudolf Merkel, Udo Seifert, Kheya Sengupta and Ana Smith, Phys. Rev. X 4, 021023 (2014) .

39. Adaptive Amphiphilic Dendrimer-Based Nanoassemblies as Robust and Versatile siRNA Delivery Systems, Liu, Xiaoxuan; Zhou, Jiehua; Yu, Tianzhu; Chen Chao ; Cheng Qian; Sengupta Kheya; Huang Yuanyu; Li HT ; Liu Cheng; Wang Yang; Posocco Paola; Wang Menghua ; Cui Qi; Giorgio Suzanne; Fermeglia Maurizio; Qu Fanqi ; Pricl Sabrina; Shi Yanhong; Liang Zicai; Rocchi Palma; Rossi John J. ; Peng Ling, Angewandte Chemie-International Edition, 53 (44) 11822-11827 (2014).

40.Association Rates of Membrane-Coupled Cell Adhesion Molecules, Timo Bihr, Susanne Fenz, Erich Sackmann, Rudolf Merkel, Udo Seifert, Kheya Sengupta, Ana-Sunčana Smith, Biophys. J. 107 (11), L33–L36 (2014).

41.Ligand-Mediated Friction Determines Morphodynamics of Spreading T Cells, Pierre Dillard, Rajat Varma, Kheya Sengupta, Laurent Limozin Biophys. J. 107 (11) 2629–2638 (2014).

42. Crowding of receptors induces ring-like adhesions in model membranes, D. Schmidt, T. Bihr, S. Fenz, R. Merkel, U. Seifert, K. Sengupta, and A.-S. Smith. Biochimica Et Biophysica Acta-Molecular Cell Research 1853 (11) 2984-2991 (2015).

43.Size-Tunable Organic Nanodot Arrays: A Versatile Platform for Manipulating and Imaging Cells, Fuwei Pi, Pierre Dillard, Ranime Alameddine, Emmanuelle Benard, Astrid Wahl, Igor Ozerov, Anne Charrier, Laurent Limozin, and Kheya Sengupta, Nano Letters, 15 (8) 5178-5184 (2015).

44. Measuring fast stochastic displacements of bio-membranes with dynamic optical displacement spectroscopy.  C. Monzel, D. Schmidt, C. Kleusch, D. Kirchenbuechler, U. Seifert, A.-S. Smith, K. Sengupta and R. Merkel. Nature communications. 6, 8162 (2015).

45. Nano-clustering of ligands on surrogate Antigen Presenting Cells modulates T cell membrane adhesion and organization. Pierre Dillard*, Fuwei Pi*, Annemarie Lelluche, Laurent Limozin, Kheya Sengupta. Integrative Biology. 8 (3), 287-301 (2016).

46. Nanometric Thermal Fluctuations of Weakly Confined Biomembranes Measured with
Microsecond Time-Resolution. Conelia Monzel, Daniel Schmidt, Udo Seifert, Ana-Suncana Smith, Rudopl Merkel, and Kheya Sengupta. SoftMatter 12, 4755-4768 (2016).

47. Measuring Shape Fluctuations in Biological Membranes, Cornelia Monzel and Kheya Sengupta, J Phys D 29 (24), 243002 (2016). BLOGPOST

48. Ligand Nano-cluster Arrays in a Supported Lipid Bilayer, Emmanuelle Benard, Fuwei Pi, Igor Ozerov, Anne Charrier, Kheya Sengupta. J. Vis. Exp. (122), e55060, doi:10.3791/55060 (2017). http://www.jove.com/video/55060.

49. Membrane fluctuations mediate lateral interaction between cadherin bonds, Susanne Fenz, Timo Bihr, Daniel Schmidt, Rudolf Merkel, Udo Seifert, Kheya Sengupta, and Ana-Suncana Smith. Nature Physics, 13, 906–913 (2017). (In News: http://www.sciencenewsline.com/news/2017061415510095.html)

50. Printing functional protein nano-dots on soft elastomers: from transfer mechanism to cell mechanosensing, Ranime Alameddine, Astrid Wahl, Fuwei Pi, Kaoutar Bouzalmate, Laurent Limozin, Anne Charrier, and Kheya Sengupta. Nano Lett., 2017, 17 (7): 4284–4290

51. T cells on engineered substrates: the impact of TCR clustering is enhanced by LFA-1 engagement
Emmanuelle Benard, Jacques A. Nunes, Laurent Limozin and Kheya Sengupta. Front. Immunol. 2018, doi: 10.3389/fimmu.2018.02085.

52. Lamellipod Reconstruction by Three-Dimensional Reflection Interference Contrast Nanoscopy (3D-RICN). M-J. Dejardin, A. Hemmerle, A. Sadoun, Y. Hamon, P-H. Puech, K. Sengupta, and L. Limozin
Nano Lett., 2018 (DOI: 10.1021/acs.nanolett.8b03134).

53. Biphasic mechanosensitivity of TCR mediated adhesion of T lymphocytes. A. Wahl, C. Dinet, P. Dillard, P-H. Puech, L. Limozin, and K. Sengupta. PNAS (2019) https://www.pnas.org/content/early/2019/03/07/1811516116              https://www.biorxiv.org/content/early/2017/12/10/232041

54. Adhesion of Biological Membranes, K. Sengupta and A. Smith. Book Chapter in "Physics of Biological Membranes", Ed. Basserau, P. and Sens, P., Springer (2018).