Kheya Sengupta

Localisation

TPR1 - 4 étage

Grade

DR2

Fonction

chercheur

Pièce

20

sengupta.jpg

Activité

Soft-matter, Biophysics, Nano-science

Thèmes

ResearcherID: E-8629-2011 || ORCID: 0000-0002-1060-2713

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My current research is focused on adhesion of living and model cells. The adhesion of cells is a fundamental biological process with implications for functions ranging from tissue morphogenesis to immune response. Very often, the primary focus in cell adhesion research is on identifying the relevant adhesion proteins and signaling pathways. However, for a complete description, it is essential to also understand the physics underlying the processes that govern the adhesion. In order to study a complex phenomenon like cell adhesion, in-vitro experiments using cell mimetic systems are often useful since the experimental conditions can be better controlled. Moreover, the simplicity of such systems facilitates theoretical modeling. The insight gained from such bio-mimetic studies can then be applied to real cells. In my research, on one hand I try to make realistic models of adhering cells using giant unilamellar vesicles and on the other hand I study the adhesion of real cells under biomimetic conditions to assess the relevance of the vesicle models.

Recherche

Current research :

* Adhesion and fluctuations of membranes

* Adhesion and mechanics of T lymphocytes

 

Membrane fluctuations mediate lateral interaction between cadherin bonds, Susanne Fenz, Timo Bihr, Daniel Schmidt, Rudolf Merkel, Udo Seifert, Kheya Sengupta, and Ana-Suncana Smith. Nature Physics, 13, 906–913 (2017). (In News: http://www.sciencenewsline.com/news/2017061415510095.html)

50. Printing functional protein nano-dots on soft elastomers: from transfer mechanism to cell mechanosensing, Ranime Alameddine, Astrid Wahl, Fuwei Pi, Kaoutar Bouzalmate, Laurent Limozin, Anne Charrier, and Kheya Sengupta. Nano Lett., 2017, 17 (7): 4284–4290

51. T cells on engineered substrates: the impact of TCR clustering is enhanced by LFA-1 engagement
Emmanuelle Benard, Jacques A. Nunes, Laurent Limozin and Kheya Sengupta. Front. Immunol. 2018, doi: 10.3389/fimmu.2018.02085.

52. Lamellipod Reconstruction by Three-Dimensional Reflection Interference Contrast Nanoscopy (3D-RICN). M-J. Dejardin, A. Hemmerle, A. Sadoun, Y. Hamon, P-H. Puech, K. Sengupta, and L. Limozin
Nano Lett., 2018 (DOI: 10.1021/acs.nanolett.8b03134).

53. Adhesion of Biological Membranes, K. Sengupta and A. Smith. Book Chapter in "Physics of Biological Membranes", Ed. Basserau, P. and Sens, P., Springer (2018).

54. Biphasic mechanosensitivity of TCR mediated adhesion of T lymphocytes. A. Wahl, C. Dinet, P. Dillard, P-H. Puech, L. Limozin, and K. Sengupta. PNAS (2019) https://www.pnas.org/content/early/2019/03/07/1811516116              https://www.biorxiv.org/content/early/2017/12/10/232041

55. Measuring Giant Vesicle adhesion, Ana-Suncana Smith and Kheya Sengupta, in "The Giant Vesicle Book", Edited by Carlos Marquez and Rumiana Dimova, CRC Press (2019) ISBN 9781315152516.

56. Ligand Nanocluster Array Enables Artificial-Intelligence-Based Detection of Hidden Features in T-Cell Architecture, Nano Letters (2021). DOI: 10.1021/acs.nanolett.1c01073.

57. Integrin-Functionalised Giant Unilamellar Vesicles via Gel-Assisted Formation: Good Practices and Pitfalls, International Journal of Molecular Sciences (2021) DOI: 10.3390/ijms22126335.

58. On the control of dispersion interactions between biological membranes and protein coated biointerfaces, Blackwell, Robert; Hemmerle, Arnaud; Baer, Andreas; Sengupta, Kheya; Smith, Ana-Suncana. Journal of Colloid and Interface Science (2021) DOI: 10.1016/J.JCIS.2021.02.078

59.Biomechanics as driver of aggregation of tethers in adherent membranes
Soft Matter (2021). DOI: 10.1039/d1sm00921d

60. May the force be with your (immune) cells: an introduction to traction force microscopy in Immunology. F. Mustapha, K. Sengupta, P-H. Puech.
Frontiers in Immunology (2022). DOI: 10.3389/fimmu.2022.898558

61. Protocol for measuring weak cellular traction forces using well-controlled ultra-soft polyacrylamide gels, F. Mustapha, K. Sengupta, P-H. Puech. STAR Protocols (2022)
DOI: 10.1016/j.xpro.2022.101133

62. Physics of Organelle Membrane Bridging via Cytosolic Tethers is Distinct From Cell Adhesion Frontiers in Physics (2022)
DOI: 10.3389/fphy.2021.750539

63. First-Principle Coarse-Graining Framework for Scale-Free Bell-Like Association and Dissociation Rates in Thermal and Active Systems, J. A. Janes, C. Monzel, D. Schmidt, R. Merkel, Rudolf, U. Seifert, K. Sengupta, A-S. Smith. Phys. Rev. X (2022) 12 (3) 031030. https://link.aps.org/doi/10.1103/PhysRevX.12.031030

Publications

2022

First-Principle Coarse-Graining Framework for Scale-Free Bell-Like Association and Dissociation Rates in Thermal and Active Systems

Josip Augustin Janeš, Cornelia Monzel, Daniel Schmidt, Rudolf Merkel, Udo Seifert, Kheya Sengupta, Ana-Sunčana Smith

Physical Review X 12:031030 (2022)10.1103/PhysRevX.12.031030

Physics of Organelle Membrane Bridging via Cytosolic Tethers is Distinct From Cell Adhesion

Mohammad Arif Kamal, Josip Augustin Janeš, Long Li, Franck Thibaudau, Ana-Sunčana Smith, Kheya Sengupta

Frontiers in Physics 9:1-12 (2022)10.3389/fphy.2021.750539

May the force be with your (immune) cells: an introduction to traction force microscopy in Immunology

Farah Mustapha, Kheya Sengupta, Pierre-Henri Puech

Frontiers in Physics 13:898558 (2022)10.3389/fimmu.2022.898558

2021

Biomechanics as driver of aggregation of tethers in adherent membranes

Long Li, Mohammad Arif Kamal, Bernd Henning Stumpf, Franck Thibaudau, Kheya Sengupta, Ana-Sunčana Smith

Soft Matter 17:10101-10107 (2021)10.1039/d1sm00921d

Ligand Nanocluster Array Enables Artificial-Intelligence-Based Detection of Hidden Features in T-Cell Architecture

Aya Nassereddine, Ahmed Abdelrahman, Emmanuelle Benard, F. Bedu, Igor Ozerov, Laurent Limozin, Kheya Sengupta

Nano Letters 21:5606-5613 (2021)10.1021/acs.nanolett.1c01073

Biomechanics as driver of aggregation of tethers in adherent membranes

Kheya Sengupta, Long Li, Mohammad Arif Kamal, Bernd Henning Stumpf, Franck Thibaudau, Ana-Sunčana Smith

Soft Matter 17:10101-10107 (2021)10.1039/D1SM00921D

Integrin-Functionalised Giant Unilamellar Vesicles via Gel-Assisted Formation: Good Practices and Pitfalls

Kheya Sengupta, Mariem Souissi, Julien Pernier, Olivier Rossier, Gregory Giannone, Christophe Le Clainche, Emmanuèle Helfer

International Journal of Molecular Sciences 22:6335 (2021)10.3390/ijms22126335

On the control of dispersion interactions between biological membranes and protein coated biointerfaces

Kheya Sengupta, Robert Blackwell, Arnaud Hemmerle, Andreas Baer, Matthias Späth, Wolfgang Peukert, Drew Parsons, Ana-Sunčana Smith

Journal of Colloid and Interface Science 598:464-473 (2021)10.1016/j.jcis.2021.02.078

2019

Biphasic mechanosensitivity of T cell receptor-mediated spreading of lymphocytes

Astrid Wahl, Céline Dinet, Pierre Dillard, Aya Nassereddine, Pierre-Henri Puech, Laurent Limozin, Kheya Sengupta

Proceedings of the National Academy of Sciences of the United States of America 116:5908-5913 (2019)10.1073/pnas.1811516116

2018

T Cells on Engineered Substrates: The Impact of TCR Clustering Is Enhanced by LFA-1 Engagement

Emmanuelle Benard, Jacques Nunès, Laurent Limozin, Kheya Sengupta

Frontiers in Immunology 9 (2018)10.3389/fimmu.2018.02085

Lamellipod Reconstruction by Three-Dimensional Reflection Interference Contrast Nanoscopy (3D-RICN)

Marie-Julie Dejardin, Arnaud Hemmerle, Anais Sadoun, Yannick Y. Hamon, Puech Pierre-Henri, Kheya Sengupta, Laurent Limozin

Nano Letters 18:6544-6550 (2018)10.1021/acs.nanolett.8b03134

2017

Printing functional protein nano-dots on soft elastomers: from transfer mechanism to cell mechanosensing

Ranime Alameddine, Astrid Wahl, Fuwei Pi, Kaoutar Bouzalmate, Laurent Limozin, Anne Charrier, Kheya Sengupta

Nano Letters 17:4284-4290 (2017)10.1021/acs.nanolett.7b01254

Ligand Nano-cluster Arrays in a Supported Lipid Bilayer

Emmanuelle Benard, Fuwei Pi, Igor Ozerov, Anne Charrier, Kheya Sengupta

Journal of visualized experiments : JoVE 122:e55060 (2017)10.3791/55060

Membrane fluctuations mediate lateral interaction between cadherin bonds

Susanne Fenz, Timo Bihr, Daniel Schmidt, Rudolf Merkel, Udo Seifert, Kheya Sengupta, Ana-Sunčana Smith

Nature Physics 13:906-913 (2017)10.1038/NPHYS4138

2016

Nano-clustering of ligands on surrogate antigen presenting cells modulates T cell membrane adhesion and organization

Pierre Dillard, Fuwei Pi, Annemarie Lellouch, Laurent Limozin, Kheya Sengupta

Integrative biology 8:287-301 (2016)10.1039/C5IB00293A

Membrane Mediated Cooperativity Facilitates Cadherin Clustering in Model Membranes

Susanne Fenz, Timo Bihr, Daniel Schmidt, Rudolf Merkel, Kheya Sengupta, Udo Seifert, Ana-Suncana Smith

Biophysical Journal 110:190A (2016)10.1016/j.bpj.2015.11.1058

Measuring shape fluctuations in biological membranes

C. Monzel, K. Sengupta

Journal of Physics D: Applied Physics 49:243002 (2016)10.1088/0022-3727/49/24/243002

Dynamic Optical Displacement Spectroscopy to Quantify Biomembrane Bending Fluctuations

Cornelia Monzel, Daniel Schmidt, Udo Seifert, Ana-Suncana Smith, Kheya Sengupta, Rudolf Merkel

Biophysical Journal 110:487A (2016)10.1016/j.bpj.2015.11.2603

Nanometric thermal fluctuations of weakly confined biomembranes measured with microsecond time-resolution

Cornelia Monzel, Daniel Schmidt, Udo Seifert, Ana-Suncana Smith, Rudolf Merkel, Kheya Sengupta

Soft Matter 12:4755-4768 (2016)10.1039/c6sm00412a

2015

Size-Tunable Organic Nanodot Arrays: A Versatile Platform for Manipulating and Imaging Cells

Fuwei Pi, Pierre Dillard, Ranime Alameddine, Emmanuelle Benard, Astrid Wahl, Igor Ozerov, Anne Charrier, Laurent Limozin, Kheya Sengupta

Nano Letters 15:5178-5184 (2015)10.1021/acs.nanolett.5b01400

2014

Signature of a Nonharmonic Potential as Revealed from a Consistent Shape and Fluctuation Analysis of an Adherent Membrane

D. Schmidt, C. Monzel, T. Bihr, R. Merkel, U. Seifert, K. Sengupta, A.S. Smith

Physical Review X 4:021023 (2014)10.1103/PhysRevX.4.021023

2013

Nanometric Protein-Patch Arrays on Glass and Polydimethylsiloxane for Cell Adhesion Studies

Fuwei Pi, Pierre Dillard, Laurent Limozin, Anne Charrier, Kheya Sengupta

Nano Letters 13:3372-3378 (2013)10.1021/nl401696m

A bola-phospholipid containing tetrafluorophenylazido chromophore as a promising lipid probe for biomembrane photolabeling studies

Y. Xia, K. Sengupta, A. Maggiani, F. Qu, Ling Peng

Organic & Biomolecular Chemistry 11:5000-5005 (2013)

2012

Giant Vesicles as Cell Models

S.F. Fenz, K. Sengupta

Integrative Biology 4:982-995 (2012)

Mapping Fluctuations in Biomembranes Adhered to Micropatterns

C. Monzel, S.F. Fenz, M. Giesen, R. Merkel, K. Sengupta

Soft Matter 8:6128-6138 (2012)

Heavy fermion superconductor CeCu 2 Si 2 under high pressure: Multiprobing the valence crossover

Gabriel Seyfarth, A.-S. Rüetschi, K. Sengupta, A. Georges, D. Jaccard, S. Watanabe, K. Miyake

Physical Review B: Condensed Matter and Materials Physics (1998-2015) 85 (2012)10.1103/PhysRevB.85.205105

Proximity to valence transition in heavy fermion superconductor CeCu 2 Si 2 under pressure

Gabriel Seyfarth, A.-S. Rüetschi, K. Sengupta, A. Georges, D. Jaccard

EPL - Europhysics Letters 98:17012 (2012)10.1209/0295-5075/98/17012

Depth matters: cells grown on nano-porous anodic alumina respond to pore depth

S. Thakur, S. Massou, A.M. Benoliel, P. Bongrand, M. Hanbücken, K. Sengupta

Nanotechnology 23:255101 (2012)

2011

Blebbing dynamics during endothelial cell spreading

L. Norman, K. Sengupta, H. Aranda-Espinoza

European Journal of Cell Biology 90:37-48 (2011)