Kheya Sengupta

Activity

Soft-matter, Biophysics, Nano-science

Thèmes

ResearcherID: E-8629-2011 || ORCID: 0000-0002-1060-2713

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My current research is focused on adhesion of living and model cells. The adhesion of cells is a fundamental biological process with implications for functions ranging from tissue morphogenesis to immune response. Very often, the primary focus in cell adhesion research is on identifying the relevant adhesion proteins and signaling pathways. However, for a complete description, it is essential to also understand the physics underlying the processes that govern the adhesion. In order to study a complex phenomenon like cell adhesion, in-vitro experiments using cell mimetic systems are often useful since the experimental conditions can be better controlled. Moreover, the simplicity of such systems facilitates theoretical modeling. The insight gained from such bio-mimetic studies can then be applied to real cells. In my research, on one hand I try to make realistic models of adhering cells using giant unilamellar vesicles and on the other hand I study the adhesion of real cells under biomimetic conditions to assess the relevance of the vesicle models.

Recherche

Current research :

* Adhesion and fluctuations of membranes

* Adhesion and mechanics of T lymphocytes

Membrane fluctuations mediate lateral interaction between cadherin bonds, Susanne Fenz, Timo Bihr, Daniel Schmidt, Rudolf Merkel, Udo Seifert, Kheya Sengupta, and Ana-Suncana Smith. Nature Physics, 13, 906–913 (2017). (In News: http://www.sciencenewsline.com/news/2017061415510095.html)

50. Printing functional protein nano-dots on soft elastomers: from transfer mechanism to cell mechanosensing, Ranime Alameddine, Astrid Wahl, Fuwei Pi, Kaoutar Bouzalmate, Laurent Limozin, Anne Charrier, and Kheya Sengupta. Nano Lett., 2017, 17 (7): 4284–4290

51. T cells on engineered substrates: the impact of TCR clustering is enhanced by LFA-1 engagement
Emmanuelle Benard, Jacques A. Nunes, Laurent Limozin and Kheya Sengupta. Front. Immunol. 2018, doi: 10.3389/fimmu.2018.02085.

52. Lamellipod Reconstruction by Three-Dimensional Reflection Interference Contrast Nanoscopy (3D-RICN). M-J. Dejardin, A. Hemmerle, A. Sadoun, Y. Hamon, P-H. Puech, K. Sengupta, and L. Limozin
Nano Lett., 2018 (DOI: 10.1021/acs.nanolett.8b03134).

53. Adhesion of Biological Membranes, K. Sengupta and A. Smith. Book Chapter in "Physics of Biological Membranes", Ed. Basserau, P. and Sens, P., Springer (2018).

54. Biphasic mechanosensitivity of TCR mediated adhesion of T lymphocytes. A. Wahl, C. Dinet, P. Dillard, P-H. Puech, L. Limozin, and K. Sengupta. PNAS (2019) https://www.pnas.org/content/early/2019/03/07/1811516116              https://www.biorxiv.org/content/early/2017/12/10/232041

55. Measuring Giant Vesicle adhesion, Ana-Suncana Smith and Kheya Sengupta, in "The Giant Vesicle Book", Edited by Carlos Marquez and Rumiana Dimova, CRC Press (2019) ISBN 9781315152516.

56. Ligand Nanocluster Array Enables Artificial-Intelligence-Based Detection of Hidden Features in T-Cell Architecture, Nano Letters (2021). DOI: 10.1021/acs.nanolett.1c01073.

57. Integrin-Functionalised Giant Unilamellar Vesicles via Gel-Assisted Formation: Good Practices and Pitfalls, International Journal of Molecular Sciences (2021) DOI: 10.3390/ijms22126335.

58. On the control of dispersion interactions between biological membranes and protein coated biointerfaces, Blackwell, Robert; Hemmerle, Arnaud; Baer, Andreas; Sengupta, Kheya; Smith, Ana-Suncana. Journal of Colloid and Interface Science (2021) DOI: 10.1016/J.JCIS.2021.02.078

59.Biomechanics as driver of aggregation of tethers in adherent membranes
Soft Matter (2021). DOI: 10.1039/d1sm00921d

60. May the force be with your (immune) cells: an introduction to traction force microscopy in Immunology. F. Mustapha, K. Sengupta, P-H. Puech.
Frontiers in Immunology (2022). DOI: 10.3389/fimmu.2022.898558

61. Protocol for measuring weak cellular traction forces using well-controlled ultra-soft polyacrylamide gels, F. Mustapha, K. Sengupta, P-H. Puech. STAR Protocols (2022)
DOI: 10.1016/j.xpro.2022.101133

62. Physics of Organelle Membrane Bridging via Cytosolic Tethers is Distinct From Cell Adhesion Frontiers in Physics (2022)
DOI: 10.3389/fphy.2021.750539

63. First-Principle Coarse-Graining Framework for Scale-Free Bell-Like Association and Dissociation Rates in Thermal and Active Systems, J. A. Janes, C. Monzel, D. Schmidt, R. Merkel, Rudolf, U. Seifert, K. Sengupta, A-S. Smith. Phys. Rev. X (2022) 12 (3) 031030. https://link.aps.org/doi/10.1103/PhysRevX.12.031030