Marc Leonetti

Localisation

TPR1 - 3 étage

Grade

DR2

Fonction

chercheur

Pièce

G.03.25

leonetti.jpg

Thèmes

Disciplines:

  • Soft matter

  • fluid mechanics at low Reynolds number

  • nonlinear physics

  • biophysics/biomechanics

  • elasticity

Research issues:

  • physics of interfaces, membranes and shells: from structure to physical and mechanical properties
  • encapsulation
  • spatiotemporal dynamics of soft particles and their shapes, from particle scale to suspension
  • Blood: margination, shape of RBC
  • electric activity of cells

Methodologies:

experiment, numerics and theory

Techniques:

  • optical microcopy (phase, DIC, fluorescence, RICM), microfluidics, high-speed imaging, surface rheometer
  • FEM-BEM code to study the shape and the  dynamics of surfactant-laden  droplets, vesicles, polymersomes and capsules

Recherche

Shape and dynamics  of vesicles under flow  (theory/simulations)

Collaboration: P.G. Chen, M. Jaeger, M2P2, Marseille, A. Farutin, c. Misbah, LIPhy, Grenoble

Past collaboration: G. Boëdec  (IRPHE, AMU, S. Gekle (University  of Bayreuth)

A vesicle is a droplet bounded by a lipid bilayer embedded in another liquid. This system knows a long standing interest due to its ability to mimic some properties of red blood cells. But it is also a fascinating system at a theoretical point of view, thanks to its original interfacial properties: resistance to bending and local surface incompressibility.  It is one of the ways to understand how soft matter flows. We study the shape and the spatiotemporal dynamics of vesicles in flow or in electric field by a methodology associating theory and numerical simulations.  We have developed our own code based on Finite Element Method and Boundary Element Method. The instabilities are characterized in the framework of nonlinear physics and soft matter. The results can highlight some behaviors of red blood cells in microcirculation.

Margination (experiments)

Past  collaboration: A. Aouane, J. Harting (Juëlich), C. Wagner (Universitaet des Saarlandes)

In microcirculation, Red Blood Cells (RBC) flow in the main part of vessels around the centre line while White Blood Cells (WBC) and Platelets (Pt) are more gathered at the edge. This phenomenon called margination is an essential step of diapedesis as WBCs and Pts are now much closer to their zones of importance in the cases of injury or tumor for example.

Margination is not observed without flow highlighting the role of hydrodynamic interactions between cells and vessel’s wall (glycocalyx and endothelial cells). Several models have been proposed involving either viscoelasticity of plasma or aggregation of globules or differentiated hydrodynamic interactions depending on a mismatch of properties (size, stiffness, shape) between WBCs and RBCs for example. Our project is to decipher the physical mechanisms in physiological conditions at the scale of vessels and networks.

Recently, in the framework of the PhD of Revaz Chachanidze, we have shown experimentally that a difference of rigidity between healthy and rigidified RBCs leads to margination, i.e. a segregation under flow: collaboration with C. Wagner, Universitaet des Saarlandes, Germany.

Fluid interfaces (experiments/theory/numerics)

Doctorant : P. Regazzi

Collaboration: E. Rio et A. Salonen, LPS, Orsay, H. Klein du département SSP, CINaM, P.G. Chen, M. Jaeger (M2P2, AMU)

Funding: ANR

This project concerns the interfaces between two miscible fluids and the surfactants-laden interfaces. We plan to develop new tools to characterize their mechanical response to constraints. We will also study some configurations at the scale of one or several droplets which depend on the local behavior of interfaces.

Lien : SSP_InterfacesFluides

Encapsulation (experiments/theory/numerics)

Past  collaboration: D. Barthès-Biesel  (BMBI, UTC), J. Deschamps (IRPHE, AMU), C. de Loubens (LRP, Grenoble), F. Edwards-Lévy (ICMR, Reims University), Reims) M. Georgelin (IRPHE, AMU), A.-V. Salsac (BMBI, UTC)

Encapsulation is a simple way of protecting, transporting and delivering internalized principles, but also of structuring space. This concerns very diverse fields such as food, cosmetics, new materials for construction or medicine. The capsules studied are droplets bounded by a thin polymer film (shell also called membrane or skin) and immersed in a liquid. The structural and mechanical properties of such objects are still poorly known: behaviour laws, elastic and viscous moduli, rupture, dynamics, etc. Indeed, there are few experimental results. A multi-scale analysis would permit to gain insight in the physics of these smart soft particles.

A - Thin films of polymers

Polymer layers can be made of various manners. We focus on interfacial polymerization where the chemical reaction between reactants is accurately localized in space, at the oil/water interface for example. When the interface is flat, we will investigate polymerization by surface rheometry, AFM and light  diffusion to determine the multi-scale structure during the growth of the membrane and its mechanical properties. It means the elastic and viscous components of the response to external constraints.

Lien : SSP_InterfacesFluides

B - dynamics, shape, wrinkling and rupture of an elastic capsule

Doctorant: P. Regazzi

Funding: CNES

The behavior of capsules (closed elastic system) under flow is governed by the coupling at the interface between the viscous stress jump and the elastic response of the shell. In the linear regime, all the elastic models are  similar to the Hooke model. However, some capsules can sustain more than 100 % of elongation  without plasticity leading to the question of the constitutive law  (stress-strain relation) governing the material shell deformation. In the same context, we are  also interested in the rupture of capsules as a function of the cohesion of its membrane which  depends on the cohesive properties of the membrane. Beyond, a capsule can exhibit two kinds of shape instabilities under flow. On one hand, the fluid-structure interactions (mechanical equilibrium) can lead to compressive domains and then to the potential emergence of wrinkles, a shape modulation with a small wavelength compared to the capsule's size. Our aim is to decipher the physical mechanisms in the regimes near threshold and far from threshold. Beyond, we expect secondary instabilities and competition between the different branches of solutions. On the  other hand, in contrast  with experimental results, there are numerous numerical studies in literature. They have shown that capsules also experience a zoology of dynamics (oscillations for example) where the shape is modulated at the capsule's scale. We will study experimentally how the shape of a capsule evolves.

See SSP_Capsules for other works on capsules in CINaM

C – Interactions between capsules, collective behaviors

The strong deformation of capsules in flow induces more complex hydrodynamic interactions than between rigid particles. To this, it is necessary to add colloidal interactions as well as friction, key ingredients for the understanding of the rheology of rigid particle suspensions. We propose to study the implication of all these contributions to the case of binary interactions and more broadly in suspension.

 

Parcours

Other:

·       Previous fundings : ANR, ACI, Labex MEC et Tec21, CNES

·       Membre du Conseil Scientifique de l'Institut de Physique du CNRS (2024-...)

·       Treasurer of the Société Française de Physique(2017-...)

·       Trésorier de la DMC (2012-2017)

.   CNU section 28

·       Prize Young Researcher D. Guinier, French  Society of Physics

·       DEA de Physique des Liquides (master degree)

·       ENS Paris

 

Publications:

[67] P. Regazzi and M. Leonetti. Deformation and orientation of a droplet in linear flows: effect of adsorption and desorption. submitted, 2024.

[66] A. Farutin, J. Lyu, P.G. Chen, M. Leonetti, C. Misbah, Generic snaking and swirling of moving entities, submitted, 2024.

[65] J. Waillez, P. Regazzi, A. Salonen, P.G. Chen, M. Jaeger, M. Leonetti, E. Rio, Drop deformation in a planar elongational flow: impact of surfactant dynamics, submitted 2024

[64] V. Puthumana, V., P.G. Chen, P. G., M. Leonetti, M., R. Lasserre, R., & M. Jaeger, Assessment of coupled bilayer–cytoskeleton modelling strategy for red blood cell dynamics in flow. Journal of Fluid Mechanics, 979 (2024) A44.

[63] J. Lyu, P.G. Chen, A. Farutin, M. Jaeger, C. Misbah and M. Leonetti, Swirling of vesicles, shapes and dynamics in Poiseuille flow  as a model of RBC microcirculation, Phys. Rev. Fluids 8 (2023) L021602

[62] Breakups of Chitosan Microcapsules in Extensional Flow, Journal of Colloid and Interface Science 629 (2023) 445-454

[61] K. Xie and M. Leonetti, Mechanical characterization of core-shell microcapsules, CRAS (2023)

[60] Structural characterization of the interfacial self- assembly of chitosan with oppositely charged surfactant, Journal of Colloid and Interface Science 616 (2022) 911 920

[59] J. Lyu, K. Xie, R. Chachanidze, A. Kahli, G. Boedec and M. Leonetti, Dynamics of shape instability of a polymersome tether, Physics of Fluids 33 (2021) 122016

[58] S. Das, M. Jaeger, M. Leonetti, R. Thaokar and G. Chen, Effect of pulse width on the dynamics of a deflated vesicle in unipolar and bipolar pulsed electric fields, Physics of Fluids, 33 (2021) 081905

[57] H. Saidani, M. Leonetti, H. Kmita and F. Homblé, The open state selectivity of the bean seed VDAC depends on stigmasterol and ion concentration, International Journal of Molecular Sciences 22, 3024 (2021)

[56] G. Simionato, K. Hinkelman, R. Chachanidze, M. Leonetti, L. Kastner, C. Wagner and S. Quint, Red blood cell phenotyping from 3D confocal images using artificial neural networks, PLOS Computational biology 17 (2021) e1008934

[55] M. Maleki, C. de Loubens, K. Xie, E. Talansier, H. Bodiguel et M. Leonetti, Membrane emulsification for high throughput produc- tion of uniform microcapsules with tunable mechanical properties, Chem. Eng. Sci 237, 116567 (2021)

[54] J. Lyu, P. G. Chen, G. Boedec, M. Leonetti and M. Jaeger, An isogeometric boundary element method for soft particles flowing in microfluidic channels, Computers and Fluids 214 (2021) 104786

[53] P. G. Chen, J. Lyu, M. Jaeger and M. Leonetti, Shape transition and hydrodynamics of vesicles in tube flow, Phys. Rev. Fluids 5 (2020) 043602

[52] M. Degonville, G. Boedec and M. Leonetti, Oblate to prolate tran- sition of a vesicle in shear flow, EPJE 42, 116 (2019)

[51] A. Naillon, C. de Loubens, W. Chevremont, S. Rouze, M. Leo- netti and H. Bodiguel, Dynamics of particle migration in confined viscoelastic Poiseuille flows, Phys. Rev. Fluids 4 (2019) 053301

[50] A. Abay, G. Simionato, R. Chachanidze, A. Bogdanova, P. Bianchi, E. Akker, M. Lindern, M. Leonetti, G. Minetti, C. Wagner and L. Kaestner, Glutaraldehyde - a subtle tool in the investigation of healthy and pathologic red blood cells, Frontiers in Physiology, ID 450377 (2019)

[49] J. Lyu, P. G. Chen, G. Boedec, M. Leonetti and M. Jaeger, Hybrid continuous-coarse graining modeling of erythrocytes, CRAS mécanique, 346, pp 439-448 (2018)

[48] K. Xie, C. de Loubens, M. Jaeger, D. Gunes and M. Leonetti, Tunable interfacial rheological properties of soft mono-disperse po- lyelectrolytes microcapsules, Soft Matter, 36, pp 6208-6217 (2017)

[47] L. Mlayeh, E-V. Krammer, M. Leonetti, M. Prévost and F. Hom- blé, The mitochondrial VDAC recruits phasphatidylethanolamine lipids for its proper functioning, BBA-Bioenergetics 1858, pp 786- 794 (2017)

[46] J. Gubspun, C. de Loubens, R. Trozzo, M. Jaeger, M. Georgelin, J. Deschamps and M. Leonetti, Flow induced by a microcapsule in a cylindrical capillary, Fluid Dyn. Research 49, 035501 (2017)

[45] G. Martrou, M. Leonetti, D. Gigmes and T. Trimaille, Straight- forward preparation of surface modified electrospun microfibers as suitable supports for protein immobilization, Polym. Chem. 8, 1790 (2017) (IF = 5.687)

[44] G. Boedec, M. Leonetti and M. Jaeger, Isogeometric FEM-BEM simulations of capsules and vesicles dynamics in Stokes flow, J. Comp. Phys. 342, 117-138 (2017)

[43] F. Homblé, H. Kmita, H. Saidani and M. Leonetti, Plant VDAC permeability: molecular basis and role in oxidative stress, in Molecular Basis for Mitochondrial Signaling edited by Tatiana Rostovtseva, Springer Biological and Medical Physics, Biomedical Engineering (2017)

[42] H. Saidani, E-V. Krammer, M. Leonetti, D. Grobys, H. Kmita, M. Prevost and F. Homble, Towards understanding of plant mitochon- drial VDAC proteins: an overview of common bean (Phaseolus) VDAC proteins, AIMS Biophysics 4, 43-62 (2017)

[41] J. Gounley, G. Boedec, M. Jaeger and M. Leonetti, Influence of surface viscosity on droplets in shear flow, J. Fluid Mech. 791, 464-494 (2016).

[40] C. de Loubens, J. Deschamps, F. Edwards-Lévy et M. leonetti, Tanktreading of microcapsules in shear flow, J. Fluid Mech. 789, 750-767 (2016).

[39] A. Guckenberger, M. Schraml, P. G. Chen, M. Leonetti and S. Gekle, On the bending algorithms for soft objects in flows, Com- puter Physics Communications 207, 1-23 (2016)

[38] J. Gubspun, P-Y. Gires, C. de Loubens, D. Barthes-Biesel, J. Des- champs, M. Georgelin, M. Leonetti, E. Leclerc, F. Edwards-Lévy and A-V. Salsac, Characterization of the mechanical properties of cross-linked serum albumin microcapsules: effect of size and pro- tein concentration, Colloid Polym. Sci. 294, 1381- 1389 (2016).

[37] M. Dionzou, A. Mozere, C. Roux, B. Lonetti, J.-D. Marty, C. Min- gotaud, P. Joseph, D. Goudouneche, B. Payre, M. Leonetti and A.-F. Mingotaud, Comparison of methods for the fabrication and the characterization of polymer self-assemblies : what are the im- portant parameters ?, Soft Matter 12, 2166-2176 (2016).

[36] C. de Loubens, J. Deschamps, G. Boedec and M. Leonetti, Stret- ching of capsules in an elongation flow, a route to constitutive law, J. Fluid Mech. 767, R3 (2015).

[35] R. Trozzo, G. Boedec, M. Leonetti and M. Jaeger, Axisymmetric Boundary Element Method for vesicles in a capillary, J. Comp. Phys. 289, 62-82 (2015)

[34] C. deLoubens, J.Deschamps, M.Georgelin, A.Charrier, F.Edwards-Lévy and M. Leonetti, Mechanical characterization of cross-linked serum albumin microcapsules, Soft Matter 10, 4561-4568 (2014)

[33] G. Boedec, M. Jaeger and M. Leonetti, Pearling instability of a cylindrical vesicle, J. Fluid Mech. 743, 262-279 (2014)

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Publications

2024

Assessment of coupled bilayer-cytoskeleton modelling strategy for red blood cell dynamics in flow

V Puthumana, Paul G. Chen, M Leonetti, R Lasserre, M Jaeger

Journal of Fluid Mechanics 979:A44 (2024)10.1017/jfm.2023.1092

2023

Breakups of Chitosan microcapsules in extensional flow

Revaz Chachanidze, Kaili Xie, Jinming Lyu, Marc Jaeger, Marc Leonetti

Journal of Colloid and Interface Science 629:445-454 (2023)10.1016/j.jcis.2022.08.169

Swirling of vesicles: Shapes and dynamics in Poiseuille flow as a model of RBC microcirculation

Jinming Lyu, Paul G. Chen, Alexander Farutin, Marc Jaeger, Chaouqi Misbah, Marc Leonetti

Physical Review Fluids 8:L021602 (2023)10.1103/PhysRevFluids.8.L021602

Mechanical characterization of core-shell microcapsules

Kaili Xie, Marc Leonetti

Comptes Rendus. Mécanique 351:1-20 (2023)10.5802/crmeca.148

2022

Structural characterization of the interfacial self-assembly of chitosan with oppositely charged surfactant

Revaz Chachanidzea, Kaili Xie, Hanna Massaad, Denis C.D. Roux, Marc Léonetti, Clément de Loubens

Journal of Colloid and Interface Science 616:911-920 (2022)10.1016/j.jcis.2022.01.143

2021

Effect of pulse width on the dynamics of a deflated vesicle in unipolar and bipolar pulsed electric fields

Sudip Das, Marc Jaeger, Marc Leonetti, Rochish M. Thaokar, Paul G. Chen

Physics of Fluids 33:081905 (2021)10.1063/5.0057168

Dynamics of pearling instability in polymersomes: the role of shear membrane viscosity and spontaneous curvature

J Lyu, K Xie, R Chachanidze, A Kahli, Gwenn Boedec, Marc Leonetti

Physics of Fluids 33:122016 (2021)10.1063/5.0075266

An isogeometric boundary element method for soft particles flowing in microfluidic channels

Jinming Lyu, Paul G. Chen, G. Boedec, M. Leonetti, Marc Jaeger

Computers and Fluids 214:104786 (2021)10.1016/j.compfluid.2020.104786

Membrane emulsification for the production of suspensions of uniform microcapsules with tunable mechanical properties

Mehdi Maleki, Clément de Loubens, Kaili Xie, Emeline Talansier, Hugues Bodiguel, Marc Léonetti

Chemical Engineering Science 237 (2021)10.1016/j.ces.2021.116567

The Open State Selectivity of the Bean Seed VDAC Depends on Stigmasterol and Ion Concentration

Hayet Saidani, Marc Léonetti, Hanna Kmita, Fabrice Homblé

International Journal of Molecular Sciences 22:3034 (2021)10.3390/ijms22063034

2020

Shape transition and hydrodynamics of vesicles in tube flow

Paul G. Chen, J M Lyu, M Jaeger, M. Leonetti

Physical Review Fluids 5:043602 (2020)10.1103/PhysRevFluids.5.043602

2019

Glutaraldehyde – A Subtle Tool in the Investigation of Healthy and Pathologic Red Blood Cells

Asena Abay, Greta Simionato, Revaz Chachanidze, Anna Bogdanova, Laura Hertz, Paola Bianchi, Emile van den Akker, Marieke von Lindern, Marc Léonetti, Giampaolo Minetti, Christian Wagner, Lars Kaestner

Frontiers in Physiology 10 (2019)10.3389/fphys.2019.00514

Oblate to prolate transition of a vesicle under flow

Maximilien Degonville, Gwenn Boedec, Marc Leonetti

European Physical Journal E: Soft matter and biological physics (2019)10.1140/epje/i2019-11881-0

Dynamics of particle migration in confined viscoelastic Poiseuille flows

Antoine Naillon, Clément de Loubens, William Chèvremont, Samuel Rouze, Marc Leonetti, Hugues Bodiguel

Physical Review Fluids 4:053301 (2019)10.1103/PhysRevFluids.4.053301

2018

Hybrid continuum–coarse-grained modeling of erythrocytes

Jinming Lyu, Paul G. Chen, Gwenn Boedec, Marc Leonetti, Marc Jaeger

Comptes Rendus Mécanique 346:439-448 (2018)10.1016/j.crme.2018.04.015

2017

Isogeometric FEM-BEM simulations of drop, capsule and vesicle dynamics in Stokes flow

Gwenn Boedec, Marc Leonetti, Marc Jaeger

Journal of Computational Physics 342:117-138 (2017)10.1016/j.jcp.2017.04.024

Perturbations of the flow induced by a microcapsule in a capillary tube

Jonathan Gubspun, Marc Georgelin, Julien Deschamps, Marc Leonetti, Clément de Loubens, Roberto Trozzo

Fluid Dynamics Research 49:035501 (2017)10.1088/1873-7005/aa6270

One-step preparation of surface modified electrospun microfibers as suitable supports for protein immobilization

Guillaume Martrou, Marc Leonetti, Didier Gigmes, Thomas Trimaille

Polymer Chemistry 8:1790-1796 (2017)10.1039/c6py02086k

Interfacial rheological properties of self-assembling biopolymer microcapsules

Kaili Xie, Clement de Loubens, Frédéric Dubreuil, Deniz Gunes, Marc Jaeger, Marc Leonetti

Soft Matter 13:6208-6217 (2017)10.1039/C7SM01377A

2016

Comparison of methods for the fabrication and the characterization of polymer self-assemblies

M. Dionzou, A. Morère, Clément Roux, Barbara Lonetti, Jean-Daniel Marty, Christophe Mingotaud, Pierre Joseph, D. Goudouneche, B. Payre, M. Leonetti, Anne-Françoise Mingotaud

Soft Matter 12:2166-2176 (2016)10.1039/c5sm01863c

Tank-treading of microcapsules in shear flow

Clément de Loubens, Julien Deschamps, Florence Edwards-Lévy, Marc Leonetti

Journal of Fluid Mechanics 789:750-767 (2016)10.1017/jfm.2015.758

Influence of surface viscosity on droplets in shear flow

J. Gounley, G. Boedec, Marc Jaeger, M. Leonetti

Journal of Fluid Mechanics 791:464- 494 (2016)10.1017/jfm.2016.39

Characterization of the mechanical properties of cross-linked serum albumin microcapsules: effect of size and protein concentration

Jonathan Gubspun, Pierre-Yves Gires, Clement de Loubens, Dominique Barthès-Biesel, Julien Deschamps, Marc Georgelin, Marc Leonetti, Eric Leclerc, Florence Edwards-Lévy, Anne-Virginie Salsac

Colloid and Polymer Science 294:1381-1389 (2016)10.1007/s00396-016-3885-8

On the bending algorithms for soft objects in flows

Achim Guckenberger, Marcel P. Schrame, Paul G. Chen, Marc Leonetti, Stephan Gekle

Computer Physics Communications 207:1-23 (2016)10.1016/j.cpc.2016.04.018

2015

Stretching of capsules in an elongation flow, a route to constitutive law

Clement de Loubens, Julien Deschamps, Gwenn Boedec, Marc Leonetti

Journal of Fluid Mechanics 767:R3 (2015)10.1017/jfm.2015.69

Axisymmetric Boundary Element Method for vesicles in a capillary

R. Trozzo, G. Boedec, M. Leonetti, M. Jaeger

Journal of Computational Physics 289:62-82 (2015)10.1016/j.jcp.2015.02.022

2014

Pearling instability of a cylindrical vesicle

Gwenn Boedec, Marc Jaeger, Marc Leonetti

Journal of Fluid Mechanics 743:262-279 (2014)10.1017/jfm.2014.34

Mechanical characterization of cross-linked serum albumin microcapsules

Clément de Loubens, Julien Deschamps, Marc Georgelin, Anne Charrier, Florence Edwards-Levy, Marc Leonetti

Soft Matter 10:4561-4568 (2014)10.139/C4SM00349G

2013

Production of human antibodies by in vitro immunization using a fusion protein containing the transcriptional transactivator of HIV-1

Mazhoura Ait Mebarek, A. Wijkhuisen, Karine Adel-Patient, P. Lamourette, M. Léonetti, H. Volland

Journal of Immunological Methods 396:96-106 (2013)10.1016/j.jim.2013.07.015

Breathing instability in biological cells, patterns of membrane proteins

Marc Leonetti, Gwenn Boedec, Marc Jaeger

Discontinuity, Nonlinearity, and Complexity 2:75-84 (2013)10.5890/DNC.2012.12.001