Departement PIV

Nanocapteurs et séquenceurs

Responsable : A. Charrier, C. Fauquet, D. Tonneau


X-ray nanosensors

Principal investigators: C. Fauquet & D. Tonneau
PostDoc S. Debnath

We develop fibered X-ray nanosensors for two main applications in Oncology (collaboration with Paoli-Calmettes Institute and CRCM Marseille) and fundamental physics (collaboration with ANL, IL USA). The active part of these sensors is integrated at the tip of a pulled optical fiber. High sensitivity and lateral resolution (20 nm demonstrated) is expected. We also develop a new generation of material characterization tools coupling Scanning Probe Microscopy and X-ray Spectrometry. The general goal is to get simultaneously sample topography and chemical mapping at high lateral resolution.

Engineering and properties of supported lipid layers and their utilization in bio/chemo and ion sensors

Principle Investigator: A. Charrier

Supported lipid layers are very interesting materials with numerous unique intrinsic properties that can be used in the development of sensors: they self-assemble into mono- or by- layers with a thickness of a few nanometers on various substrates; lipids present lateral fluidity in the plane of the layer; they form natural dielectrics.



  • we chemically engineer lipids and lipid layers to make the layers functional and with high degree of mechanical and dielectric stability
  • we study the formation of lipid layers on inorganic and organic substrates and their  interfaces
  • we develop sensors based on field effect transistor technologies using engineered lipid layers as ultra-thin gate dielectrics





Single molecule T-Jump (Thibaudau, Marmri PhD)

Kinetic measurements are among the most important experimental tools for elucidating the mechanisms of biochemical reactions. The sequences and orders of the elementary reaction steps and their dynamics can be deduced from kinetic data and their dependence on experimental conditions or perturbations. A rapid temperature variation (T-Jump, iT-Jump) allows to study many biologically relevant processes or their elementary steps that occur at time scales of microsecond. In recent years, advances in nano-fabrication techniques have enabled the production of nanoscale devices that probe a single molecule present in a solution at concentrations relevant to biology. Such devices have been used to perform single molecule studies of biological processes such as DNA polymerization or protein-protein interaction. However, they are not designed to allow a brief variation in the temperature of the molecule for the study of processes and / or to activate them thermally on a microsecond scale. We are developing an instrument based on nano-devices to produce microsecond temperature flashes and optically analyze a single molecule.


DNA polymerase mechanisms and polymerization of DNA under thermal flashes. (Thibaudau, Marmri PhD)




Photocatalytical properties of NiO nanofilms doped with Ba

S. Boulila, M. Ghamnia, A. Boukhachem, A. Ouhaibi, M. Chakhoum, C. Fauquet, V. Heresanu, D. Tonneau

Philosophical Magazine Letters 100:283-293 (2020)10.1080/09500839.2020.1760389

Femtomolar detection of Cu2+ ions in solution using super-Nernstian FET-sensor with a lipid monolayer as top-gate dielectric

A. Kenaan, F. Brunel, J.-M. Raimundo, Anne Charrier

Sensors and Actuators B: Chemical 316:128147 (2020)10.1016/j.snb.2020.128147

Electrolyte-gated-organic field effect transistors functionalized by lipid monolayers with tunable pH sensitivity for sensor applications

Tin Phan Nguy, Ryoma Hayakawa, Volkan Kilinc, Matthieu Petit, Yemineni S L V Narayana, Masayoshi Higuchi, Jean-Manuel Raimundo, Anne Charrier, Yutaka Wakayama

Applied Physics Express 13:011005 (2020)10.7567/1882-0786/ab5322


Investigation of Some Physical Properties of Cobalt Doped MoO3 Nanofilms and Their Effects on the Degradation of the Methylene Blue Solution under UV Illumination

N. Benameur, A. Boukhachem, M. Ghamnia, M. Chakhoum, M. Dahamni, C. Fauquet

International Journal of Chemical Engineering and Applications 10:33-39 (2019)10.18178/ijcea.2019.10.2.736

High spatial resolution inorganic scintillator detector for high energy X-ray beam at small field irradiation

S.B.C Debnath, Agnes Tallet, S. Lavandier, Sree Bash, Chandra Debnath, C. Fauquet, Agnès Tallet, Anthony Gonçalves, S Ebastien Lavandier, F. Jandard, D. Tonneau, Julien Darréon

Medical Physics (2019)10.1002/MP.14002

Structure of a Zn monolayer on Ag(111) and Ag(110) substrates: an AES, LEED and STM study

Maradj Hicham, Carole Fauquet, Mostefa Ghamnia, Benedicte Ealet, Nabil Rochdi, Sébastien Vizzini, Jean-Paul Biberian, Bernard Aufray, Haik Jamgotchian

Surface Science (2019)10.1016/j.susc.2019.02.007

Self-organization of red blood cell suspensions under confined 2D flows

Cécile Iss, Dorian Midou, Alexis Moreau, Delphine Held, Anne Charrier, Simon Mendez, Annie Viallat, Emmanuèle Helfer

Soft Matter (2019)10.1039/C8SM02571A

Novel and innovative interface as potential active layer in Chem-FET sensor devices for the specific sensing of Cs

Volkan Kilinc, Catherine Henry-De-Villeneuve, Tin Phan Nguy, Yutaka Wakayama, Anne Charrier, Jean-Manuel Raimundo

ACS Applied Materials & Interfaces 11:47635-47641 (2019)10.1021/acsami.9b18188

Stable operation of water-gated organic field-effect transistor depending on channel flatness, electrode metals and surface treatment

Tin Phan Nguy, Ryoma Hayakawa, Volkan Kilinc, Matthieu Petit, Jean-Manuel Raimundo, Anne Charrier, Yutaka Wakayama

Japanese Journal of Applied Physics 58:SDDH02 (2019)10.7567/1347-4065/ab09d2


Nanometrology of Biomass for Bioenergy: The Role of Atomic Force Microscopy and Spectroscopy in Plant Cell Characterization

Anne Charrier, Aude Lereu, Rubye Farahi, Brian Davison, Ali Passian

Frontiers in Energy Research 6:11 (2018)10.3389/fenrg.2018.00011

Facile Nanopatterning of PEDOT:PSS Thin Films

Mohammed Elmahmoudy, Anne Charrier, George Malliaras, Sébastien Sanaur

Advanced Materials Technologies 3:1700344 (2018)10.1002/admt.201700344

Ultra-thin supported lipid monolayer with unprecedented mechanical and dielectric properties

Ahmad Kenaan, Racha El Zein, Volkan Kilinc, Sébastien Lamant, Jean-Manuel Raimundo, Anne Charrier

Advanced Functional Materials 1801024 (2018)10.1002/adfm.201801024

The effect of strontium doping on structural and morphological properties of ZnO nanofilms synthesized by ultrasonic spray pyrolysis method

A. Ouhaibi, M. Ghamnia, A. Dahamni, V. Heresanu, C. Fauquet, D. Tonneau

Journal of Science: Advanced Materials and Devices 3:29-36 (2018)10.1016/j.jsamd.2018.01.004


Printing functional protein nano-dots on soft elastomers: from transfer mechanism to cell mechanosensing

Ranime Alameddine, Astrid Wahl, Fuwei Pi, Kaoutar Bouzalmate, Laurent Limozin, Anne Charrier, Kheya Sengupta

Nano Letters 17:4284-4290 (2017)10.1021/acs.nanolett.7b01254

Ligand Nano-cluster Arrays in a Supported Lipid Bilayer

Emmanuelle Benard, Fuwei Pi, Igor Ozerov, Anne Charrier, Kheya Sengupta

Journal of visualized experiments : JoVE 122:e55060 (2017)10.3791/55060

Tailoring the electrochemical and mechanical properties of PEDOT : pss films for bioelectronics

Mohammed Elmahmoudy, Sahika Inal, Anne Charrier, Ilke Uguz, George Malliaras, Sébastien Sanaur

Macromolecular Materials and Engineering 302 (2017)10.1002/mame.201600497

Plasticity, elasticity, and adhesion energy of plant cell walls: nanometrology of lignin loss using atomic force microscopy

R. Farahi, Anne Charrier, A. Tolbert, A. Lereu, A. Ragauskas, B. Davison, A. Passian

Scientific Reports 7 (2017)10.1038/s41598-017-00234-4

High Aspect Ratio Sub-Micrometer Channels Using Wet Etching: Application to the Dynamics of Red Blood Cell Transiting through Biomimetic Splenic Slits

Priya Gambhire, Scott Atwell, Cécile Iss, Frédéric Bedu, Igor Ozerov, Catherine Badens, Emmanuèle Helfer, Annie Viallat, Anne Charrier

Small 13:1700967 (2017)10.1002/smll.201700967

Investigation of some physical properties of ZnO nanofilms synthesized by micro-droplet technique

N. Hamzaoui, A. Boukhachem, M. Ghamnia, C. Fauquet

Results in Physics 7:1950-1958 (2017)10.1016/j.rinp.2017.06.005

Ultracompact x-ray dosimeter based on scintillators coupled to a nano-optical antenna

Zhihua Xie, Hichem Maradj, Miguel-Angel Suarez, Lydie Viau, Virginie Moutarlier, Claudine Filiatre, Carole Fauquet, Didier Tonneau, Thierry Grosjean

Optics Letters 42:1361-1364 (2017)10.1364/OL.42.001361


Subpicomolar Iron Sensing Platform Based on Functional Lipid Monolayer Microarrays

Ahmad Kenaan, Tuyen D. Nguyen, Herve Dallaporta, Jean-Manuel Raimundo, Anne M. Charrier

Analytical Chemistry 88:3804-3809 (2016)10.1021/acs.analchem.5b04834