Département PIV

Mécanique de biofluides actifs

Responsable : A. Charrier, E. Helfer, A. Viallat


Physics of cellular senescence

Principal investigators: E. Helfer
PhD Student: C. Jebane

Cellular senescence (aging) is a normal process defined as the progressive decline of all cellular functions ending with cell cycle arrest. In particular conditions, senescence can occur prematurely and induce premature aging, such as in the most severe case of Progeria. We study the process of premature aging specifically associated with alterations of the lamina, a component of the nuclear envelope (NE) which ensures the mechanical stability and rigidity of the nucleus. Using microfluidic devices we investigate the effects of lamina mutations on mechanical and rheological properties of nuclei and cells, and how they modify the mechanotransductive response of cells to mechanical stimuli.

Dynamics of individual red blood cells in the microcirculation .

Principal investigators: A. Viallat, A. Charrier, E. Helfer,
PhD Student: A. Moreau
Postdocs: M. Sahun

The efficient and sustainable circulation of blood cells in the vasculature is a physical tour de force. First, blood cells never form aggregates susceptible to clog blood vessels. Second, red blood cells combine robustness and high deformability to squeeze through the smallest capillaries. Although evolution has succeeded in finding the suitable physical parameters that ensure a proper vascular circulation, we are today unable to predict, understand, and artificially reproduce individual and collective behaviors of blood cells in the vasculature.

We study how the mechanical properties of individual red blood cells govern their motion in shear flow

We develop a microfluidic device containing submicron slits mimicking interendothelial splenic slits to study the stringent mechanical fitness test that red blood cells pass in the spleen.

We develop mechanical markers for the clinical follow-up of sickle cell disease and pathologies affecting red blood cell deformability.

Physics of the mucociliary clearance.

Principal investigators: A. Viallat,
Postdoc: E. Loiseau

The airway epithelium is protected by the active transport at its surface of a layer of a complex fluid, the mucus. It is powered by the beats of billions of microscopic cilia carried by epithelial cells. These beats present a strong long-range orientational order but the physical mechanisms leading to the emergence and maintain of this collective dynamic order of ciliary beats is not known. They involve the mechanical cilia-mucus coupling mediated by long-range hydrodynamic interactions, and the active mechanotransductive response of ciliary beats.

We study the long-range self-organization of ciliary activity and mucus flow patterns in reconstituted bronchial epithelium.



Mechanical adaptation of monocytes in model lung capillary networks

Jules Dupire, Pierre-Henri Puech, Emmanuèle Helfer, Annie Viallat

Proceedings of the National Academy of Sciences of the United States of America (2020)

Hydrodynamic model of directional ciliary-beat organization in human airways

Simon Gsell, Etienne Loiseau, Umberto D’ortona, Annie Viallat, Julien Favier

Scientific Reports 10 (2020)10.1038/s41598-020-64695-w

Femtomolar detection of Cu2+ ions in solution using super-Nernstian FET-sensor with a lipid monolayer as top-gate dielectric

A. Kenaan, F. Brunel, J.-M. Raimundo, Anne Charrier

Sensors and Actuators B: Chemical 316:128147 (2020)10.1016/j.snb.2020.128147

Active mucus–cilia hydrodynamic coupling drives self-organization of human bronchial epithelium

Etienne Loiseau, Simon Gsell, Aude Nommick, Charline Jomard, Delphine Gras, Pascal Chanez, Umberto D’ortona, Laurent Kodjabachian, Julien Favier, Annie Viallat

Nature Physics (2020)10.1038/s41567-020-0980-z

Electrolyte-gated-organic field effect transistors functionalized by lipid monolayers with tunable pH sensitivity for sensor applications

Tin Phan Nguy, Ryoma Hayakawa, Volkan Kilinc, Matthieu Petit, Yemineni S L V Narayana, Masayoshi Higuchi, Jean-Manuel Raimundo, Anne Charrier, Yutaka Wakayama

Applied Physics Express 13:011005 (2020)10.7567/1882-0786/ab5322

Molecular and Mechanobiological Pathways Related to the Physiopathology of FPLD2

Alice-Anaïs Varlet, Emmanuèle Helfer, Catherine Badens

Cells 9:1947 (2020)10.3390/cells9091947


Self-organization of red blood cell suspensions under confined 2D flows

Cécile Iss, Dorian Midou, Alexis Moreau, Delphine Held, Anne Charrier, Simon Mendez, Annie Viallat, Emmanuèle Helfer

Soft Matter (2019)10.1039/C8SM02571A

Novel and innovative interface as potential active layer in Chem-FET sensor devices for the specific sensing of Cs

Volkan Kilinc, Catherine Henry-De-Villeneuve, Tin Phan Nguy, Yutaka Wakayama, Anne Charrier, Jean-Manuel Raimundo

ACS Applied Materials & Interfaces 11:47635-47641 (2019)10.1021/acsami.9b18188

Stable operation of water-gated organic field-effect transistor depending on channel flatness, electrode metals and surface treatment

Tin Phan Nguy, Ryoma Hayakawa, Volkan Kilinc, Matthieu Petit, Jean-Manuel Raimundo, Anne Charrier, Yutaka Wakayama

Japanese Journal of Applied Physics 58:SDDH02 (2019)10.7567/1347-4065/ab09d2


Nanometrology of Biomass for Bioenergy: The Role of Atomic Force Microscopy and Spectroscopy in Plant Cell Characterization

Anne Charrier, Aude Lereu, Rubye Farahi, Brian Davison, Ali Passian

Frontiers in Energy Research 6:11 (2018)10.3389/fenrg.2018.00011

Facile Nanopatterning of PEDOT:PSS Thin Films

Mohammed Elmahmoudy, Anne Charrier, George Malliaras, Sébastien Sanaur

Advanced Materials Technologies 3:1700344 (2018)10.1002/admt.201700344

Ultra-thin supported lipid monolayer with unprecedented mechanical and dielectric properties

Ahmad Kenaan, Racha El Zein, Volkan Kilinc, Sébastien Lamant, Jean-Manuel Raimundo, Anne Charrier

Advanced Functional Materials 1801024 (2018)10.1002/adfm.201801024

Spatiotemporal organization of cilia drives multiscale mucus swirls in model human bronchial epithelium

Mustapha-Kamel Khelloufi, Etienne Loiseau, Marc Jaeger, Nicolas Molinari, Pascal Chanez, Delphine Gras, Annie Viallat

Scientific Reports 8:2447 (2018)10.1038/s41598-018-20882-4

When giant vesicles mimic red blood cell's dynamics: swinging of two-phase vesicles in shear flow

Simon Tusch, Etienne Loiseau, Al-Hair Al-Halifa, Kamel Khelloufi, Emmanuèle Helfer, Annie Viallat

Physical Review Fluids 3 (2018)10.1103/PhysRevFluids.3.123605


Printing functional protein nano-dots on soft elastomers: from transfer mechanism to cell mechanosensing

Ranime Alameddine, Astrid Wahl, Fuwei Pi, Kaoutar Bouzalmate, Laurent Limozin, Anne Charrier, Kheya Sengupta

Nano Letters 17:4284-4290 (2017)10.1021/acs.nanolett.7b01254

Ligand Nano-cluster Arrays in a Supported Lipid Bilayer

Emmanuelle Benard, Fuwei Pi, Igor Ozerov, Anne Charrier, Kheya Sengupta

Journal of visualized experiments : JoVE 122:e55060 (2017)10.3791/55060

Tailoring the electrochemical and mechanical properties of PEDOT : pss films for bioelectronics

Mohammed Elmahmoudy, Sahika Inal, Anne Charrier, Ilke Uguz, George Malliaras, Sébastien Sanaur

Macromolecular Materials and Engineering 302 (2017)10.1002/mame.201600497

Plasticity, elasticity, and adhesion energy of plant cell walls: nanometrology of lignin loss using atomic force microscopy

R. Farahi, Anne Charrier, A. Tolbert, A. Lereu, A. Ragauskas, B. Davison, A. Passian

Scientific Reports 7 (2017)10.1038/s41598-017-00234-4

High Aspect Ratio Sub-Micrometer Channels Using Wet Etching: Application to the Dynamics of Red Blood Cell Transiting through Biomimetic Splenic Slits

Priya Gambhire, Scott Atwell, Cécile Iss, Frédéric Bedu, Igor Ozerov, Catherine Badens, Emmanuèle Helfer, Annie Viallat, Anne Charrier

Small 13:1700967 (2017)10.1002/smll.201700967


Subpicomolar Iron Sensing Platform Based on Functional Lipid Monolayer Microarrays

Ahmad Kenaan, Tuyen D. Nguyen, Herve Dallaporta, Jean-Manuel Raimundo, Anne M. Charrier

Analytical Chemistry 88:3804-3809 (2016)10.1021/acs.analchem.5b04834