Physics of cellular senescence
Principal investigators: E. Helfer
PhD Student: C. Jebane
Cellular senescence (aging) is a normal process defined as the progressive decline of all cellular functions ending with cell cycle arrest. In particular conditions, senescence can occur prematurely and induce premature aging, such as in the most severe case of Progeria. We study the process of premature aging specifically associated with alterations of the lamina, a component of the nuclear envelope (NE) which ensures the mechanical stability and rigidity of the nucleus. Using microfluidic devices we investigate the effects of lamina mutations on mechanical and rheological properties of nuclei and cells, and how they modify the mechanotransductive response of cells to mechanical stimuli.
Dynamics of individual red blood cells in the microcirculation .
The efficient and sustainable circulation of blood cells in the vasculature is a physical tour de force. First, blood cells never form aggregates susceptible to clog blood vessels. Second, red blood cells combine robustness and high deformability to squeeze through the smallest capillaries. Although evolution has succeeded in finding the suitable physical parameters that ensure a proper vascular circulation, we are today unable to predict, understand, and artificially reproduce individual and collective behaviors of blood cells in the vasculature.
We study how the mechanical properties of individual red blood cells govern their motion in shear flow
We develop a microfluidic device containing submicron slits mimicking interendothelial splenic slits to study the stringent mechanical fitness test that red blood cells pass in the spleen.
We develop mechanical markers for the clinical follow-up of sickle cell disease and pathologies affecting red blood cell deformability.
Physics of the mucociliary clearance.
The airway epithelium is protected by the active transport at its surface of a layer of a complex fluid, the mucus. It is powered by the beats of billions of microscopic cilia carried by epithelial cells. These beats present a strong long-range orientational order but the physical mechanisms leading to the emergence and maintain of this collective dynamic order of ciliary beats is not known. They involve the mechanical cilia-mucus coupling mediated by long-range hydrodynamic interactions, and the active mechanotransductive response of ciliary beats.
We study the long-range self-organization of ciliary activity and mucus flow patterns in reconstituted bronchial epithelium.
Cécile Iss, Dorian Midou, Alexis Moreau, Delphine Held, Anne Charrier, Simon Mendez, Annie Viallat, Emmanuèle Helfer
Soft Matter (2019)10.1039/C8SM02571A
Novel and innovative interface as potential active layer in Chem-FET sensor devices for the specific sensing of Cs
Volkan Kilinc, Catherine Henry-De-Villeneuve, Tin Phan Nguy, Yutaka Wakayama, Anne Charrier, Jean-Manuel Raimundo
ACS Applied Materials & Interfaces (2019)
Stable operation of water-gated organic field-effect transistor depending on channel flatness, electrode metals and surface treatment
Tin Nguy, Ryoma Hayakawa, Volkan Kilinc, Matthieu Petit, Jean-Manuel Raimundo, Anne Charrier, Yutaka Wakayama
Japanese Journal of Applied Physics 58:SDDH02 (2019)10.7567/1347-4065/ab09d2
Nanometrology of Biomass for Bioenergy: The Role of Atomic Force Microscopy and Spectroscopy in Plant Cell Characterization
Anne Charrier, Aude Lereu, Rubye Farahi, Brian Davison, Ali Passian
Frontiers in Energy Research 6:11 (2018)10.3389/fenrg.2018.00011
Mohammed Elmahmoudy, Anne Charrier, George Malliaras, Sébastien Sanaur
Advanced Materials Technologies 3:1700344 (2018)10.1002/admt.201700344
Ahmad Kenaan, Racha El Zein, Volkan Kilinc, Sébastien Lamant, Jean-Manuel Raimundo, Anne Charrier
Advanced Functional Materials (2018)10.1002/adfm.201801024
Spatiotemporal organization of cilia drives multiscale mucus swirls in model human bronchial epithelium
Mustapha-Kamel Khelloufi, Etienne Loiseau, Marc Jaeger, Nicolas Molinari, Pascal Chanez, Delphine Gras, Annie Viallat
Scientific Reports 8:2447 (2018)10.1038/s41598-018-20882-4
Simon Tusch, Etienne Loiseau, Al-Hair Al-Halifa, Kamel Khelloufi, Emmanuèle Helfer, Annie Viallat
Physical Review Fluids 3 (2018)10.1103/PhysRevFluids.3.123605
Printing functional protein nano-dots on soft elastomers: from transfer mechanism to cell mechanosensing
Ranime Alameddine, Astrid Wahl, Fuwei Pi, Kaoutar Bouzalmate, Laurent Limozin, Anne Charrier, Kheya Sengupta
Nano Letters 17:4284-4290 (2017)10.1021/acs.nanolett.7b01254
Emmanuelle Benard, Fuwei Pi, Igor Ozerov, Anne Charrier, Kheya Sengupta
Journal of visualized experiments : JoVE 122:e55060 (2017)10.3791/55060
Mohammed Elmahmoudy, Sahika Inal, Anne Charrier, Ilke Uguz, George Malliaras, Sébastien Sanaur
Macromolecular Materials and Engineering 302 (2017)10.1002/mame.201600497
Plasticity, elasticity, and adhesion energy of plant cell walls: nanometrology of lignin loss using atomic force microscopy
R. Farahi, Anne Charrier, A. Tolbert, A. Lereu, A. Ragauskas, B. Davison, A. Passian
Scientific Reports 7 (2017)10.1038/s41598-017-00234-4
High Aspect Ratio Sub-Micrometer Channels Using Wet Etching: Application to the Dynamics of Red Blood Cell Transiting through Biomimetic Splenic Slits
Priya Gambhire, Scott Atwell, Cécile Iss, Frédéric Bedu, Igor Ozerov, Catherine Badens, Emmanuèle Helfer, Annie Viallat, Anne Charrier
Small 13:1700967 (2017)10.1002/smll.201700967
Ahmad Kenaan, Tuyen D. Nguyen, Herve Dallaporta, Jean-Manuel Raimundo, Anne M. Charrier
Analytical Chemistry 88:3804-3809 (2016)10.1021/acs.analchem.5b04834